Human Succinate dehydrogenase cytochrome b560 subunit, mitochondrial, SDHC ELISA Kit | SDHC elisa kit

Human Succinate dehydrogenase cytochrome b560 subunit, mitochondrial, SDHC ELISA Kit

Cat. Num. AAA2602659

• Gene Names: SDHC; CYBL; PGL3; QPS1; SDH3; CYB560
• Reactivity: Human
• Synonyms: Succinate dehydrogenase cytochrome b560 subunit; mitochondrial; SDHC; Human Succinate dehydrogenase cytochrome b560 subunit; SDHC ELISA Kit; SDHC elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.

• Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant SDHC concentration
• Assay Type: Sandwich
• Detection Range: 10 ng/ml-0.156 ng/ml
• Sensitivity: 0.05 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SDHC elisa kit

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human SDHC monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 78711820
• NCBI GeneID: 6391
• NCBI Accession #: NP_001030588.1
• NCBI GenBank Nucleotide #: NM_001035511.1
• UniProt Accession #: Q99643; O75609; Q3C259; Q3C2D8; Q3C2H4; Q5VTH3
• Molecular Weight: 18,610 Da
• NCBI Official Full Name: succinate dehydrogenase cytochrome b560 subunit, mitochondrial isoform 2
• NCBI Official Synonym Full Names: succinate dehydrogenase complex, subunit C, integral membrane protein, 15kDa
• NCBI Official Symbol: SDHC
• NCBI Official Synonym Symbols: CYBL; PGL3; QPS1; SDH3; CYB560
• NCBI Protein Information: succinate dehydrogenase cytochrome b560 subunit, mitochondrial; integral membrane protein CII-3b; succinate dehydrgenase cytochrome b; cytochrome B large subunit of complex II; succinate dehydrogenase 3, integral membrane subunit; succinate-ubiquinone oxidoreductase cytochrome B large subunit; succinate dehydrogenase complex, subunit C, integral membrane protein, 15kD
• UniProt Protein Name: Succinate dehydrogenase cytochrome b560 subunit, mitochondrial
• Protein Family: Succinate dehydrogenase 2 membrane
• UniProt Gene Name: SDHC
• UniProt Synonym Gene Names: CYB560; SDH3; QPs1; CYBL
• UniProt Entry Name: C560_HUMAN

NCBI Description

This gene encodes one of four nuclear-encoded subunits that comprise succinate dehydrogenase, also known as mitochondrial complex II, a key enzyme complex of the tricarboxylic acid cycle and aerobic respiratory chains of mitochondria. The encoded protein is one of two integral membrane proteins that anchor other subunits of the complex, which form the catalytic core, to the inner mitochondrial membrane. There are several related pseudogenes for this gene on different chromosomes. Mutations in this gene have been associated with paragangliomas. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2013]

Uniprot Description

SDHC: Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Defects in SDHC are the cause of paragangliomas type 3 (PGL3). A neural crest tumor usually derived from the chromoreceptor tissue of a paraganglion. Paragangliomas are most commonly located in the head and neck region, specifically at the carotid bifurcation, the jugular foramen, the vagal nerve, and in the middle ear. Defects in SDHC are a cause of paraganglioma and gastric stromal sarcoma (PGGSS); also known as Carney- Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance. Belongs to the cytochrome b560 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Energy Metabolism - oxidative phosphorylation; Membrane protein, multi-pass; Mitochondrial; Carbohydrate Metabolism - citrate (TCA) cycle; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: mitochondrial respiratory chain complex II; mitochondrion; mitochondrial inner membrane; integral to membrane

Molecular Function: electron carrier activity; metal ion binding; heme binding; succinate dehydrogenase activity

Biological Process: cellular metabolic process; tricarboxylic acid cycle; aerobic respiration

Disease: Gastrointestinal Stromal Tumor; Paraganglioma And Gastric Stromal Sarcoma; Paragangliomas 3