Serine Peptidase Inhibitor Kunitz Type 2 Recombinant Protein | SPINT2 recombinant protein

Recombinant Serine Peptidase Inhibitor Kunitz Type 2 (SPINT2)

Cat. Num. AAA2033497

• Gene Names: SPINT2; PB; Kop; HAI2; DIAR3; HAI-2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Serine Peptidase Inhibitor Kunitz Type 2; Recombinant Serine Peptidase Inhibitor Kunitz Type 2 (SPINT2); HAI2; Kop; PB; Placental Bikunin; Hepatocyte growth factor activator inhibitor type 2; SPINT2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: ADR ERSIHDFCLV SKVVGRCRASMPRWWYNVTD GSCQLFVYGG CDGNSNNYLT KEECLKKCAT VTENATGDLATSRNAADSSV PSAPRRQDSE DHSSDMFNYE EYCTANAVTG PCRASFPRWYFDVERNSCNN FIYGGCRGNK NSYRSEEACM LRCFRQQENP PLPLGSK
• Sequence Length: 252

• Applicable Applications for SPINT2 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 49.2kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Ala28~Lys197
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and GST-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 15277534
• NCBI GeneID: 10653
• Molecular Weight: 21,828 Da
• NCBI Official Full Name: Serine peptidase inhibitor, Kunitz type, 2
• NCBI Official Synonym Full Names: serine peptidase inhibitor, Kunitz type, 2
• NCBI Official Symbol: SPINT2
• NCBI Official Synonym Symbols: PB; Kop; HAI2; DIAR3; HAI-2
• NCBI Protein Information: kunitz-type protease inhibitor 2
• UniProt Protein Name: Kunitz-type protease inhibitor 2
• Protein Family: Kunitz-type protease inhibitor
• UniProt Gene Name: SPINT2
• UniProt Synonym Gene Names: HAI2; KOP; HAI-2
• UniProt Entry Name: SPIT2_HUMAN

NCBI Description

This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. This gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

SPINT2: Inhibitor of HGF activator. Also inhibits plasmin, plasma and tissue kallikrein, and factor XIa. Defects in SPINT2 are the cause of diarrhea type 3 (DIAR3); also known as congenital sodium diarrhea (CSD). DIAR3 is a rare, inherited diarrhea of infancy. A diagnosis of DIAR3 is made on the findings of a life-threatening secretory diarrhea, severe metabolic acidosis, and hyponatremia secondary to extraordinarily high fecal losses of sodium, with low or normal excretion of urinary sodium, in the absence of infectious, autoimmune, and endocrine causes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Inhibitor

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cytoplasm; extracellular region; integral to membrane; plasma membrane

Molecular Function: endopeptidase inhibitor activity; serine-type endopeptidase inhibitor activity

Biological Process: cell motility; establishment and/or maintenance of cell polarity; negative regulation of cell-cell adhesion; neural tube closure

Disease: Diarrhea 3, Secretory Sodium, Congenital, With Or Without Other Congenital Anomalies