Western Blot (WB)
(Western blot analysis of EYA3 expression in Hela (A), HT29 (B), mouse eye (C), mouse testis (D), rat brain (E), rat liver (F) whole cell lysates.)
Rabbit EYA3 Polyclonal Antibody | anti-EYA3 antibody
Anti-EYA3 Antibody
Western Blot (WB)
(Western blot analysis of EYA3 expression in Hela (A), HT29 (B), mouse eye (C), mouse testis (D), rat brain (E), rat liver (F) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of EYA3 expression in Hela (A), HT29 (B), mouse eye (C), mouse testis (D), rat brain (E), rat liver (F) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of EYA3 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of EYA3 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3' UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2013]
Uniprot Description
EYA3: Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1, and seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity. May be involved in development of the eye. Belongs to the HAD-like hydrolase superfamily. EYA family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor)
Chromosomal Location of Human Ortholog: 1p36
Cellular Component: centrosome; cytoplasm; nucleoplasm; nucleus; transcription factor complex
Molecular Function: metal ion binding; protein binding; protein tyrosine phosphatase activity
Biological Process: anatomical structure morphogenesis; double-strand break repair; histone dephosphorylation; multicellular organismal development; positive regulation of DNA repair; regulation of transcription, DNA-dependent; response to ionizing radiation; transcription, DNA-dependent; visual perception
Research Articles on EYA3
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Product Notes
The EYA3 eya3 (Catalog #AAA8245734) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-EYA3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EYA3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1/500 - 1/2000; IHC: 1/50 - 1/200. Researchers should empirically determine the suitability of the EYA3 eya3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EYA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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