Rabbit anti-Human SSRP1 Polyclonal Antibody | anti-SSRP1 antibody

SSRP1, CT (SSRP1, FACT80, FACT complex subunit SSRP1, Chromatin-specific transcription elongation factor 80kD subunit, Facilitates chromatin transcription complex 80kD subunit, Facilitates chromatin transcription complex subunit SSRP1, Recombination signa

Cat. Num. AAA6351369

• Gene Names: SSRP1; FACT; T160; FACT80
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SSRP1; Polyclonal Antibody; CT (SSRP1; FACT80; FACT complex subunit SSRP1; Chromatin-specific transcription elongation factor 80kD subunit; Facilitates chromatin transcription complex 80kD subunit; Facilitates chromatin transcription complex subunit SSRP1; Recombination signa; Recombination signal sequence recognition protein 1; Structure-specific recognition protein 1; T160; anti-SSRP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-SSRP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SSRP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 489-518 amino acids from the C-terminal region of human SSRP1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SSRP1 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the SSRP1 antibody detected the SSRP1 protein (arrow).)

Related Product Information for anti-SSRP1 antibody

The protein encoded by this gene is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63. An alternatively spliced transcript variant of this gene has been described, but its full-length nature is not known.

Product Categories/Family for anti-SSRP1 antibody

Antibodies; Chromatin Proteins

References

Zeng, S.X., et al. Mol. Cell. Biol. 30(4):935-947(2010). Hautbergue, G.M., et al. Curr. Biol. 19(22):1918-1924(2009). Hu, J., et al. J. Virol. 83(21):11051-11063(2009). Kumari, A., et al. J. Cell. Biochem. 108(2):508-518(2009). Okada, M., et al. Mol. Biol. Cell 20(18):3986-3995(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4507241
• NCBI GeneID: 6749
• NCBI Accession #: NP_003137.1
• NCBI GenBank Nucleotide #: NM_003146.2
• UniProt Accession #: Q08945; Q5BJG8
• Molecular Weight: 81,075 Da
• NCBI Official Full Name: FACT complex subunit SSRP1
• NCBI Official Synonym Full Names: structure specific recognition protein 1
• NCBI Official Symbol: SSRP1
• NCBI Official Synonym Symbols: FACT; T160; FACT80
• NCBI Protein Information: FACT complex subunit SSRP1; hSSRP1; FACTp80; cisplatin-DNA SSRP; FACT 80 kDa subunit; high mobility group box; structure-specific recognition protein 1; facilitates chromatin remodeling 80 kDa subunit; recombination signal sequence recognition protein 1
• UniProt Protein Name: FACT complex subunit SSRP1
• UniProt Gene Name: SSRP1
• UniProt Synonym Gene Names: FACT80; FACT 80 kDa subunit; FACTp80; hSSRP1
• UniProt Entry Name: SSRP1_HUMAN

Uniprot Description

SSRP1: a subunit of the chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63.

Protein type: Transcription initiation complex; Transcription, coactivator/corepressor; RNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: nucleoplasm; cytoplasm; nucleolus; chromosome; nucleus

Molecular Function: protein binding; DNA binding; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; chromatin remodeling; regulation of transcription, DNA-dependent; viral reproduction; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; gene expression; DNA repair; DNA replication