Sheep Pulmonary surfactant-associated protein B ELISA Kit | SP-B elisa kit

Sheep Pulmonary surfactant-associated protein B (SP-B) ELISA Kit

Cat. Num. AAA285192

• Gene Names: SFTPB; SP-B; SFTP3
• Reactivity: Sheep
• Synonyms: Pulmonary surfactant-associated protein B; Sheep Pulmonary surfactant-associated protein B (SP-B) ELISA Kit; PSP-B; SFTB3; SFTP3; SMDP1; SP-B; OTTHUMP00000203253|Pulmonary surfactant-associated protein B; 18kD|surfactant; pulmonary-associated protein B; SP-B elisa kit

• Reactivity: Sheep
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sheep SP-B. No significant cross-reactivity or interference between Sheep SP-B and analogues was observed.
• Sequence Length: 369

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for SP-B elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate SP-B in samples. An antibody specific for SP-B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySP-B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SP-B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SP-B bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 126723505
• NCBI GeneID: 100009194
• NCBI Accession #: NP_001075812.1
• NCBI GenBank Nucleotide #: NM_001082343.1
• Molecular Weight: 40,610 Da
• NCBI Official Full Name: pulmonary surfactant-associated protein B
• NCBI Official Symbol: SFTPB
• NCBI Official Synonym Symbols: SP-B; SFTP3
• NCBI Protein Information: pulmonary surfactant-associated protein B
• UniProt Protein Name: Pulmonary surfactant-associated protein B
• Protein Family: Pulmonary surfactant-associated protein
• UniProt Gene Name: SFTPB
• UniProt Synonym Gene Names: SFTP3; SP-B
• UniProt Entry Name: PSPB_RABIT

Uniprot Description

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.