Human Pulmonary surfactant-associated protein B ELISA Kit | SFTPB elisa kit

Human Pulmonary surfactant-associated protein B ELISA Kit

Cat. Num. AAA761993

• Gene Names: SFTPB; SP-B; SFTP3
• Reactivity: Human
• Synonyms: Pulmonary surfactant-associated protein B; Human Pulmonary surfactant-associated protein B ELISA Kit; SPB (Pulmonary Surfactant Associated Protein B)/SFTPB/SP-B/SFTP3/Pulmonary surfactant-associated proteolipid SPL (Phe)/18 kDa pulmonary-surfactant protein/6 kDa protein; SFTPB elisa kit

• Reactivity: Human
• Sequence Length: 369

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.781-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for SFTPB elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human SPBantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human SPB antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SPB amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman SPBcan be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 126723505
• NCBI GeneID: 100009194
• NCBI Accession #: NP_001075812.1
• NCBI GenBank Nucleotide #: NM_001082343.1
• Molecular Weight: 40,610 Da
• NCBI Official Full Name: pulmonary surfactant-associated protein B
• NCBI Official Symbol: SFTPB
• NCBI Official Synonym Symbols: SP-B; SFTP3
• NCBI Protein Information: pulmonary surfactant-associated protein B
• UniProt Protein Name: Pulmonary surfactant-associated protein B
• Protein Family: Pulmonary surfactant-associated protein
• UniProt Gene Name: SFTPB
• UniProt Synonym Gene Names: SFTP3; SP-B
• UniProt Entry Name: PSPB_RABIT

Uniprot Description

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.