Mouse CD20 Monoclonal Antibody | anti-MS4A1 antibody

Anti-CD20 Antibody, Mouse Monoclonal

Cat. Num. AAA8104553

• Gene Names: MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
• Reactivity: Human, Cynomolgus
• Applications: Immunohistochemistry
• Purity: Protein A
• Synonyms: CD20; Monoclonal Antibody; Anti-CD20 Antibody; Mouse Monoclonal; CD20/MS4A1 Antibody; Mouse MAb; membrane-spanning 4-domains; subfamily A; member 1; Anti-B1 Antibody; Anti-Bp35 Antibody; Anti-CVID5 Antibody; Anti-LEU-16 Antibody; Anti-MS4A1 Antibody; Anti-MS4A2 Antibody; Anti-S7 Antibody; anti-MS4A1 antibody

• Host: Mouse
• Reactivity: Human, Cynomolgus
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 06
• Specificity: Human CD20
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MS4A1 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:30-1:100
• Immunogen: Recombinant Human CD20/MS4A1 Protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD20/MS4A1 (rh CD20/MS4A1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining CD20 in human tonsil with mouse monoclonal antibody at 1:60 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining CD20 in human lymphoma with mouse monoclonal antibody at 1:60 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining CD20 in cynomolgus spleen with mouse monoclonal antibody at 1:60 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining CD20 in cynomolgus lymph node with mouse monoclonal antibody at 1:60 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-MS4A1 antibody

CD20 (membrane-spanning 4-domains, subfamily A, member 1), also known as MS4A1, is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. CD20/MS4A1 is expressed in all stages of B cell development except the first and last. CD20/MS4A1 is present from pre-pre B cells through memory cells, but not on either pro-B cells or plasma cells. It is a B-lymphocyte surface molecule that plays a role in the development and differentiation of B-cells into plasma cells. CD20/MS4A1may be involved in the regulation of B-cell activation and proliferation. Defects in CD20/MS4A1 are the cause of immunodeficiency common variable type 5(CVID5). CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections, and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells are usually in the normal range but can be very low.

Product Categories/Family for anti-MS4A1 antibody

Cancer Drug Targets

References

Tedder TF, et al. (1988) Isolation and structure of a cDNA encoding the B1 (CD20) cell-surface antigen of human B lymphocytes. Proc Natl Acad Sci. 85(1): 208-12.
Cragg MS, et al. (2005) The biology of CD20 and its potential as a target for mAb therapy. Curr Dir Autoimmun. 8: 140-74..
Polyak MJ, et al. (2003) A cholesterol-dependent CD20 epitope detected by the FMC7 antibody. Leukemia. 17(7): 1384-9.

NCBI and Uniprot Product Information

• NCBI GI #: 23110987
• NCBI GeneID: 931
• NCBI Accession #: NP_068769.2
• NCBI GenBank Nucleotide #: NM_021950.3
• UniProt Accession #: P11836; P08984; Q13963; A6NMS4; B4DT24
• Molecular Weight: 33077 MW
• NCBI Official Full Name: B-lymphocyte antigen CD20
• NCBI Official Synonym Full Names: membrane spanning 4-domains A1
• NCBI Official Symbol: MS4A1
• NCBI Official Synonym Symbols: B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
• NCBI Protein Information: B-lymphocyte antigen CD20
• UniProt Protein Name: B-lymphocyte antigen CD20
• Protein Family: CD209 antigen
• UniProt Gene Name: MS4A1
• UniProt Synonym Gene Names: CD20
• UniProt Entry Name: CD20_HUMAN

NCBI Description

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

MS4A1: This protein may be involved in the regulation of B-cell activation and proliferation. Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the MS4A family.

Protein type: Membrane protein, integral; Cell surface; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: extracellular space; integral to plasma membrane; external side of plasma membrane

Molecular Function: epidermal growth factor receptor binding

Biological Process: B cell proliferation; humoral immune response

Disease: Immunodeficiency, Common Variable, 5