Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human XPNPEP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Jurkat cell lysate Lane 2 : HeLa cell lysate Lane 3 : MCF7 cell lysate)
Mouse anti-Human XPNPEP1 Monoclonal Antibody | anti-XPNPEP1 antibody
XPNPEP1 antibody
Gene Names
XPNPEP1; APP1; SAMP; XPNPEP; XPNPEPL; XPNPEPL1
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Synonyms
XPNPEP1; Monoclonal Antibody; XPNPEP1 antibody; Xaa-Pro aminopeptidase 1 isoform 1; APP1; SAMP; XPNPEP; XPNPEPL; XPNPEPL1; anti-XPNPEP1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT9C7
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
666
Applicable Applications for anti-XPNPEP1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Antigen Species
Human
Immunogen
Recombinant human XPNPEP1 (1-623aa) purified from E. coli
Clone Note
Anti-human XPNPEP1 mAb, clone AT9C7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human XPNPEP1 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human XPNPEP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Jurkat cell lysate Lane 2 : HeLa cell lysate Lane 3 : MCF7 cell lysate)
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human XPNPEP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Jurkat cell lysate Lane 2 : HeLa cell lysate Lane 3 : MCF7 cell lysate)
Immunofluorescence (IF)
(CC/IF analysis of XPNPEP1 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).)
Immunofluorescence (IF)
(CC/IF analysis of XPNPEP1 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).)
Related Product Information for anti-XPNPEP1 antibody
XPNPEP1 also known as Xaa-Pro aminopeptidase 1 isoform 1, is encodes the cytosolic form of a metalloaminopeptidase that catalyzes the cleavage of the N-terminal amino acid adjacent to a proline residue. This product may play a role in degradation and maturation of tachykinins, neuropeptides, and peptide hormones.
Product Categories/Family for anti-XPNPEP1 antibody
References
Cottrell G.S., et al. (2000) Biochemistry. 39: 15121-15128. ; Li X., et al. (2008) J. Biol. Chem. 283: 22858-22866.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72,107 Da
NCBI Official Full Name
xaa-Pro aminopeptidase 1 isoform 1
NCBI Official Synonym Full Names
X-prolyl aminopeptidase 1
NCBI Official Symbol
XPNPEP1
NCBI Official Synonym Symbols
APP1; SAMP; XPNPEP; XPNPEPL; XPNPEPL1
NCBI Protein Information
xaa-Pro aminopeptidase 1
UniProt Protein Name
Xaa-Pro aminopeptidase 1
UniProt Gene Name
XPNPEP1
UniProt Synonym Gene Names
sAmp
NCBI Description
This gene encodes the cytosolic form of a metalloaminopeptidase that catalyzes the cleavage of the N-terminal amino acid adjacent to a proline residue. The gene product may play a role in degradation and maturation of tachykinins, neuropeptides, and peptide hormones. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]
Uniprot Description
Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro.
Research Articles on XPNPEP1
Similar Products
Product Notes
The XPNPEP1 xpnpep1 (Catalog #AAA200468) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The XPNPEP1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's XPNPEP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the XPNPEP1 xpnpep1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "XPNPEP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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