Suppressors Of Cytokine Signaling 2 Recombinant Protein | SOCS2 recombinant protein

Recombinant Suppressors Of Cytokine Signaling 2 (SOCS2)

Cat. Num. AAA2031289

• Gene Names: SOCS2; CIS2; SSI2; Cish2; SSI-2; SOCS-2; STATI2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Suppressors Of Cytokine Signaling 2; Recombinant Suppressors Of Cytokine Signaling 2 (SOCS2); SOCS2 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MTLRCLEPSG NGGEGTRSQW GTAGSAEEPS PQAARLAKAL RELGQTGWYW GSMTVNEAKE KLKEAPEGTF LIRDSSHSDY LLTISVKTSA GPTNLRIEYQ DGKFRLDSII CVKSKLKQFD SVVHLIDYYV QMCKDKRTGPEAPRNGTVHL YLTKPLYTSA PSLQHLCRLT INKCTGAIWG LPLPTRLKDY LEEYKFQV
• Sequence Length: 198

• Applicable Applications for SOCS2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 26.0kDa
• Accurate Molecular Mass (KD): 26kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, T7 tag
• Organism Species: Homo sapiens (Human)
• Fragment: Pro336~Arg540
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 394582059
• NCBI GeneID: 8835
• NCBI Accession #: NP_001257397.1
• NCBI GenBank Nucleotide #: NM_001270468.1
• Molecular Weight: 22,172 Da
• NCBI Official Full Name: suppressor of cytokine signaling 2
• NCBI Official Synonym Full Names: suppressor of cytokine signaling 2
• NCBI Official Symbol: SOCS2
• NCBI Official Synonym Symbols: CIS2; SSI2; Cish2; SSI-2; SOCS-2; STATI2
• NCBI Protein Information: suppressor of cytokine signaling 2
• UniProt Protein Name: Suppressor of cytokine signaling 2
• Protein Family: Suppressor of cytokine signaling
• UniProt Gene Name: SOCS2
• UniProt Synonym Gene Names: CIS2; SSI2; STATI2; SOCS-2; CIS-2; SSI-2
• UniProt Entry Name: SOCS2_HUMAN

NCBI Description

This gene encodes a member of the suppressor of cytokine signaling (SOCS) family. SOCS family members are cytokine-inducible negative regulators of cytokine receptor signaling via the Janus kinase/signal transducer and activation of transcription pathway (the JAK/STAT pathway). SOCS family proteins interact with major molecules of signaling complexes to block further signal transduction, in part, by proteasomal depletion of receptors or signal-transducing proteins via ubiquitination. The expression of this gene can be induced by a subset of cytokines, including erythropoietin, GM-CSF, IL10, interferon (IFN)-gamma and by cytokine receptors such as growth horomone receptor. The protein encoded by this gene interacts with the cytoplasmic domain of insulin-like growth factor-1 receptor (IGF1R) and is thought to be involved in the regulation of IGF1R mediated cell signaling. This gene has pseudogenes on chromosomes 20 and 22. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]

Uniprot Description

SOCS2: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin- protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Interacts with IGF1 receptor, prolactin receptor and growth hormone (GH) receptor. Associates with the Elongin BC complex. By a subset of cytokines, including EPO/erythropoietin and CSF2/GM-CSF. High expression in heart, placenta, lung, kidney and prostate.

Chromosomal Location of Human Ortholog: 12q

Cellular Component: cytoplasm; cytosol

Molecular Function: insulin-like growth factor receptor binding; protein binding; growth hormone receptor binding; protein kinase inhibitor activity; SH3/SH2 adaptor activity; JAK pathway signal transduction adaptor activity

Biological Process: positive regulation of signal transduction; cytokine and chemokine mediated signaling pathway; regulation of signal transduction; negative regulation of protein kinase activity; protein ubiquitination; negative regulation of insulin receptor signaling pathway; regulation of cell growth; JAK-STAT cascade; negative regulation of JAK-STAT cascade; response to estradiol stimulus; negative regulation of apoptosis; aging; cellular response to hormone stimulus