Western Blot (WB)
(Western Blot: Sample: Bovine Testis lysate; Primary Ab: 3ug/ml Rabbit Anti-Bovine CYP11A1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Cattle Cytochrome P450 11A1 Polyclonal Antibody | anti-CYP11A1 antibody
Polyclonal Antibody to Cytochrome P450 11A1 (CYP11A1)
Gene Names
CYP11A1; CYP11A; CYPXIA1; P450(scc)
Reactivity
Cattle
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Synonyms
Cytochrome P450 11A1; Polyclonal Antibody; Polyclonal Antibody to Cytochrome P450 11A1 (CYP11A1); anti-CYP11A1 antibody
Host
Rabbit
Reactivity
Cattle
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-CYP11A1 antibody
WB; IHC; ICC; IP.
Application Notes
Western blotting: 0.5-2 ug/mL; 1:500-2000
Immunohistochemistry: 5:20 ug/mL; 1:50-200
Immunocytochemistry: 5-20 ug/mL; 1:50-200
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5:20 ug/mL; 1:50-200
Immunocytochemistry: 5-20 ug/mL; 1:50-200
Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Organism Species
Bos taurus Bovine (Cattle)
Traits
Liquid
Immunogen
Recombinant CYP11A1(Val391~Gln515) expressed in E.coli.
Cross Reactivity
Bovine
Conjugated Antibody
The APC-Cy7 conjugated antibody version of this item is also available as catalog #MBS2071815
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles
Store at 4°C for frequent use
Aliquot and store at -20°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is les than 5% within the expiration date under appropiate storage condition.
Store at 4°C for frequent use
Aliquot and store at -20°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is les than 5% within the expiration date under appropiate storage condition.
Western Blot (WB)
(Western Blot: Sample: Bovine Testis lysate; Primary Ab: 3ug/ml Rabbit Anti-Bovine CYP11A1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Bovine Testis lysate; Primary Ab: 3ug/ml Rabbit Anti-Bovine CYP11A1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60,333 Da
NCBI Official Full Name
cholesterol side-chain cleavage enzyme, mitochondrial
NCBI Official Symbol
CYP11A1
NCBI Official Synonym Symbols
CYP11A; CYPXIA1; P450(scc)
NCBI Protein Information
cholesterol side-chain cleavage enzyme, mitochondrial
UniProt Protein Name
Cholesterol side-chain cleavage enzyme, mitochondrial
Protein Family
UniProt Gene Name
CYP11A1
Uniprot Description
Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.
Research Articles on CYP11A1
Similar Products
Product Notes
The CYP11A1 cyp11a1 (Catalog #AAA2025969) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Cytochrome P450 11A1 (CYP11A1) reacts with Cattle and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome P450 11A1 can be used in a range of immunoassay formats including, but not limited to, WB; IHC; ICC; IP. Western blotting: 0.5-2 ug/mL; 1:500-2000 Immunohistochemistry: 5:20 ug/mL; 1:50-200 Immunocytochemistry: 5-20 ug/mL; 1:50-200Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the CYP11A1 cyp11a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome P450 11A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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