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SDS-Page

SNRPG recombinant protein

SNRPG, 1-76aa, Human, His tag, E Coli

Gene Names
SNRPG; SMG; Sm-G
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
SNRPG; 1-76aa; Human; His tag; E Coli; Small nuclear ribonucleoprotein polypeptide G; Sm-G; SMG; SNRPG recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 50% glycerol, 0.1M NaCl
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MSKAHPPELK KFMDKKLSLK LNGGRHVQGI LRGFDPFMNLVIDECVEMAT SGQQNNIGMV VIRGNSIIML EALERV
Sequence Length
76
Applicable Applications for SNRPG recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for SNRPG recombinant protein
Small nuclear ribonucleoprotein polypeptide G, also known as SNRPG, belongs to the snRNP Sm proteins family. There are at least seven isoforms, B/B, E, F, G, D1, D2, and D3. This class of common proteins plays an essential role in the biogenesis of the snRNPs. The genes encoding human Sm G map to chromosomes 2. In addition, these proteins represent the major targets for the so-called anti-Sm auto-antibodies which are for systemic lupus erythematosus (SLE). One class of these autoantibodies reacts specifically with native Sm E-F-G complexes. Recombinant human SNRPG protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for SNRPG recombinant protein
References
Hermann H., et al. (1995) EMBO J. 14 (9):2076-88.; Heinrichs V., et al. (1992) J Mol Biol. 227 (1):15-28.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10.6 kDa (96aa), confirmed by MALDI-TOF
NCBI Official Full Name
small nuclear ribonucleoprotein G isoform a
NCBI Official Synonym Full Names
small nuclear ribonucleoprotein polypeptide G
NCBI Official Symbol
SNRPG
NCBI Official Synonym Symbols
SMG; Sm-G
NCBI Protein Information
small nuclear ribonucleoprotein G
UniProt Protein Name
Small nuclear ribonucleoprotein G
UniProt Gene Name
SNRPG
UniProt Synonym Gene Names
PBSCG; snRNP-G; Sm-G; SmG

NCBI Description

The protein encoded by this gene is a component of the U1, U2, U4, and U5 small nuclear ribonucleoprotein complexes, precursors of the spliceosome. The encoded protein may also be a part of the U7 small nuclear ribonucleoprotein complex, which participates in the processing of the 3' end of histone transcripts. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

Uniprot Description

Core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing.

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Product Notes

The SNRPG snrpg (Catalog #AAA203833) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's SNRPG can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the SNRPG snrpg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MSKAHPPELK KFMDKKLSLK LNGGRHVQGI LRGFDPFMNL VIDECVEMAT SGQQNNIGMV VIRGNSIIML EALERV. It is sometimes possible for the material contained within the vial of "SNRPG, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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