Rabbit ATP6V0A1 Polyclonal Antibody | anti-ATP6V0A1 antibody

ATP6V0A1 Antibody

Cat. Num. AAA7103638

• Gene Names: ATP6V0A1; a1; Stv1; VPP1; Vph1; ATP6N1; ATP6N1A
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: ATP6V0A1; Polyclonal Antibody; ATP6V0A1 Antibody; V-type proton ATPase 116 kDa subunit a isoform 1; V-ATPase 116 kDa isoform a1; Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit; Vacuolar adenosine triphosphatase subunit Ac116; Vacuolar proton pump subunit 1; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 1; ATP6N1; ATP6N1A; VPP1; anti-ATP6V0A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 838

• Applicable Applications for anti-ATP6V0A1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB:1:500-1:5000; IHC:1:20-1:200; IF:1:50-1:200
• Immunogen: Recombinant Human V-type proton ATPase 116 kDa subunit a isoform 1 protein (80-260AA)
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Species: Human
• Research Area: Others
• Preparation and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Rat brain tissue, Mouse brain tissueAll lanes: ATP6V0A1 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 97, 96 KDaObserved band size: 97 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7103638 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of A549 cells using MBS7103638 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-ATP6V0A1 antibody

Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 194097401
• NCBI GeneID: 535
• NCBI Accession #: NP_001123492.1
• NCBI GenBank Nucleotide #: NM_001130020.1
• UniProt Accession #: Q93050; Q8N5G7; Q9NSX0; B7Z3B7
• NCBI Official Full Name: V-type proton ATPase 116 kDa subunit a isoform 1 isoform a
• NCBI Official Synonym Full Names: ATPase H+ transporting V0 subunit a1
• NCBI Official Symbol: ATP6V0A1
• NCBI Official Synonym Symbols: a1; Stv1; VPP1; Vph1; ATP6N1; ATP6N1A
• NCBI Protein Information: V-type proton ATPase 116 kDa subunit a isoform 1; V-type proton ATPase 116 kDa subunit a
• UniProt Protein Name: V-type proton ATPase 116 kDa subunit a isoform 1
• Protein Family: V-type proton ATPase
• UniProt Gene Name: ATP6V0A1
• UniProt Synonym Gene Names: ATP6N1; ATP6N1A; VPP1; V-ATPase 116 kDa isoform a1

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three A subunit proteins and the encoded protein is associated with clathrin-coated vesicles. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle ().