SNCG recombinant protein

SNCG recombinant protein

Cat. Num. AAA515167

• Gene Names: SNCG; SR; BCSG1
• Applications: Western Blot
• Synonyms: SNCG; SNCG recombinant protein; BCSG1; PERSYN; PRSN; SR

• Host: E Coli
• Form/Format: 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Sequence Length: 855

• Applicable Applications for SNCG recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: His tag
• Expression System: E.coli
• Source Note: Recombinant full length human SNCG was expressed in E. coli cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for SNCG recombinant protein

Recombinant full length human SNCG was expressed in E. coli cells using an N-terminal His tag.

Scientific Background: SNCG or synuclein, gamma (breast cancer-specific protein 1) is a member of the synuclein family of proteins which are believed to be involved in the pathogenesis of neurodegenerative diseases. SNCG gene may also be overexpressed in ovarian cancers (1). The overexpression of SNCG may indicate breast cancer malignant progression from benign or in situ carcinoma. SNCG may also play a role in motor neurons dysfunction and cell death (2). Synuclein proteins play an important role in regulating neurotransmitter release from specific populations of midbrain dopamine neurons through mechanisms that differ from those reported in other neurons.

Product Categories/Family for SNCG recombinant protein

Microtubule/Actin Associated Proteins; Signaling Proteins - Microtubule/Actin Associated Proteins

References

1. Ji, H. et.al: Identification of a breast cancer-specific gene, BCSG1, by direct differential cDNA sequencing. Cancer Res. 57: 759-764, 1997.
2. Lavedan, C. et.al: Absence of mutation in the beta- and gamma-synuclein genes in familial autosomal dominant Parkinson's disease. DNA Research 5: 401-402, 1998.

NCBI and Uniprot Product Information

• NCBI GI #: 209693438
• NCBI GeneID: 6623
• NCBI Accession #: NP_003078.2
• NCBI GenBank Nucleotide #: NM_003087
• UniProt Accession #: O76070; O15104; Q96P61
• Molecular Weight: ~17.5 kDa
• NCBI Official Full Name: Homo sapiens synuclein, gamma (breast cancer-specific protein 1) (SNCG), mRNA
• NCBI Official Synonym Full Names: synuclein, gamma (breast cancer-specific protein 1)
• NCBI Official Symbol: SNCG
• NCBI Official Synonym Symbols: SR; BCSG1
• NCBI Protein Information: gamma-synuclein; persyn; synoretin; breast cancer-specific gene 1 protein
• UniProt Protein Name: Gamma-synuclein
• Protein Family: Gamma-synuclein
• UniProt Gene Name: SNCG
• UniProt Synonym Gene Names: BCSG1; PERSYN; PRSN; SR
• UniProt Entry Name: SYUG_HUMAN

NCBI Description

This gene encodes a member of the synuclein family of proteins which are believed to be involved in the pathogenesis of neurodegenerative diseases. Mutations in this gene have also been associated with breast tumor development. [provided by RefSeq, Jan 2010]

Uniprot Description

Function: Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases

By similarity. May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway

By similarity.

Subunit structure: May be a centrosome-associated protein.

Subcellular location: Cytoplasm › perinuclear region. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Cytoplasm › cytoskeleton › spindle. Note: Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle. Ref.7

Tissue specificity: Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung.

Post-translational modification: Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases. Ref.6

Sequence similarities: Belongs to the synuclein family.