Mouse anti-Human PIK3R1 / p85 Alpha Monoclonal Antibody | anti-PIK3R1 antibody

Mouse Monoclonal [clone 6G10] (IgG1) to Human PIK3R1 / p85 Alpha

Cat. Num. AAA249089

• Gene Names: PIK3R1; p85; AGM7; GRB1; IMD36; p85-ALPHA
• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay, ELISA
• Purity: Purified
• Synonyms: PIK3R1 / p85 Alpha; Monoclonal Antibody; Mouse Monoclonal [clone 6G10] (IgG1) to Human PIK3R1 / p85 Alpha; Anti-PIK3R1 / p85 Alpha Antibody (clone 6G10) IHC-plus; PIK3R1; p85; p85 Alpha; p85-ALPHA; PI3K regulatory subunit alpha; PI3-kinase subunit p85-alpha; GRB1; Human PIK3R1; anti-PIK3R1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6G10
• Specificity: Human PIK3R1 / p85 Alpha
• Purity/Purification: Purified
• Form/Format: PBS, 0.05% sodium azide, 0.05% protein stabilizer
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 361

• Applicable Applications for anti-PIK3R1 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: ELISA (1:10000), Flo (1:200 - 1:400), ICC (1:100), IF, IHC-P (1:200), WB (1:500 - 1:2000)
• Target Species: Human
• Immunogen Description: Purified recombinant fragment of human PIK3R1 (aa159-388) expressed in E Coli.
• Immunogen Type: Recombinant protein
• Immunogen: PIK3R1 / p85 Alpha antibody was raised against purified recombinant fragment of human PIK3R1 (aa159-388) expressed in E Coli.
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-PIK3R1 / p85 Alpha antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunofluorescence (IF)

(Immunofluorescence of HeLa cells using PIK3R1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)

Immunofluorescence (IF)

(Immunofluorescence of HepG2 cells using PIK3R1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)

Flow Cytometry (FC/FACS)

(Flow cytometry of NIH3T3 cells using PIK3R1 mouse mAb (green) and negative control (red).)

Western Blot (WB)

(Western blot of PIK3R1 mAb against human PIK3R1 recombinant protein. (Expected MW is 53.4 kDa))

ELISA (EIA)

(Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);)

Product Categories/Family for anti-PIK3R1 antibody

Family: Non-Protein Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 335057534
• NCBI GeneID: 5295
• NCBI Accession #: NP_001229395.1
• NCBI GenBank Nucleotide #: NM_001242466.1
• UniProt Accession #: P27986; Q15747; Q4VBZ7; Q53EM6; Q8IXA2; Q8N1C5; B3KT19; D3DWA0; E7EX19
• Molecular Weight: 42,838 Da
• NCBI Official Full Name: phosphatidylinositol 3-kinase regulatory subunit alpha isoform 4
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
• NCBI Official Symbol: PIK3R1
• NCBI Official Synonym Symbols: p85; AGM7; GRB1; IMD36; p85-ALPHA
• NCBI Protein Information: phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; ptdIns-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase regulatory subunit; phosphatidylinositol 3-kinase-associated p-85 alpha; phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
• UniProt Protein Name: Phosphatidylinositol 3-kinase regulatory subunit alpha
• Protein Family: Phosphatidylinositol 3-kinase regulatory
• UniProt Gene Name: PIK3R1
• UniProt Synonym Gene Names: GRB1; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha
• UniProt Entry Name: P85A_HUMAN

NCBI Description

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Uniprot Description

PIK3R1: regulatory subunit of phosphoinositide-3-kinase. Mediates binding to a subset of tyrosine-phosphorylated proteins through its SH2 domain. Acts as an adapter, mediating the association of the p110 catalytic unit of the alpha, beta and delta enzymes to the plasma membrane, where p110 phosphorylates inositol lipids. May play an additional role in the regulation of the actin cytoskeleton. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Its SH2 domains interacts with the YTHM motif of phosphorylated INSR in vitro. Defects in PIK3R1 are a cause of severe insulin resistance. Four alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Kinase, lipid; Enzyme, regulatory subunit

Chromosomal Location of Human Ortholog: 5q13.1

Cellular Component: membrane; cytoplasm; plasma membrane; intercellular junction; nucleus; phosphoinositide 3-kinase complex; cytosol

Molecular Function: insulin binding; insulin-like growth factor receptor binding; protein binding; ErbB-3 class receptor binding; insulin receptor substrate binding; neurotrophin TRKA receptor binding; phosphoinositide 3-kinase binding; phosphoinositide 3-kinase regulator activity; transcription factor binding; protein phosphatase binding; transmembrane receptor protein tyrosine kinase adaptor protein activity; insulin receptor binding

Biological Process: negative regulation of osteoclast differentiation; phosphoinositide 3-kinase cascade; viral reproduction; nerve growth factor receptor signaling pathway; protein amino acid phosphorylation; positive regulation of RNA splicing; regulation of phosphoinositide 3-kinase activity; T cell receptor signaling pathway; NFAT protein import into nucleus; positive regulation of glucose import; phosphoinositide phosphorylation; phospholipid metabolic process; positive regulation of transcription factor import into nucleus; epidermal growth factor receptor signaling pathway; platelet activation; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; protein stabilization; negative regulation of cell-matrix adhesion; positive regulation of tumor necrosis factor production; induction of apoptosis via death domain receptors; cellular response to insulin stimulus; insulin-like growth factor receptor signaling pathway; DNA damage response, signal transduction resulting in induction of apoptosis; B cell differentiation; T cell costimulation; insulin receptor signaling pathway; phosphatidylinositol biosynthetic process; cell glucose homeostasis; innate immune response; positive regulation of transcription from RNA polymerase II promoter; vascular endothelial growth factor receptor signaling pathway; blood coagulation; leukocyte migration; negative regulation of apoptosis; positive regulation of cell migration

Disease: Agammaglobulinemia 7, Autosomal Recessive; Short Syndrome; Immunodeficiency 36