Rabbit anti-Human BIRC2 / cIAP1 Polyclonal Antibody | anti-BIRC2 antibody

Rabbit Polyclonal (IgG) to Human BIRC2 / cIAP1

Cat. Num. AAA241824

• Gene Names: BIRC2; API1; MIHB; HIAP2; RNF48; cIAP1; Hiap-2; c-IAP1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Unpurified serum
• Synonyms: BIRC2 / cIAP1; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human BIRC2 / cIAP1; Anti-BIRC2 / cIAP1 Antibody IHC-plus; BIRC2; API1; C-IAP1; CIAP1; Hiap-2; IAP homolog B; HIAP2; IAP-2; RNF48; Apoptosis inhibitor 1; IAP2; MIHB; RING finger protein 48; Human BIRC2; cIAP1; anti-BIRC2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: A synthetic peptide corresponding to 361-372 of human c-IAP1 (Genbank# AAC50372).
• Purity/Purification: Unpurified serum
• Form/Format: Neat serum, 0.05% sodium azide.
• Sequence Length: 618

• Applicable Applications for anti-BIRC2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (1:250), WB (1:500); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human BIRC2 / cIAP1.
• Immunogen Type: Synthetic peptide
• Immunogen: BIRC2 / cIAP1 antibody was raised against synthetic peptide from human BIRC2 / cIAP1.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-cIAP1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:250.)

Immunohistochemistry (IHC)

(Anti-cIAP1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:250.)

Immunohistochemistry (IHC)

(Anti-cIAP1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:250.)

Immunohistochemistry (IHC)

(Anti-cIAP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:250.)

Western Blot (WB)

(Western blot detection of c-IAP1 in human lung cell lysate. using antibody at 2 µg/ml dilution.)

Related Product Information for anti-BIRC2 antibody

Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases.

Product Categories/Family for anti-BIRC2 antibody

Family: Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 4502141
• NCBI GeneID: 329
• NCBI Accession #: NP_001157.1
• NCBI GenBank Nucleotide #: NM_001166.4
• UniProt Accession #: Q13490; Q16516; Q4TTG0; B4E026
• Molecular Weight: 64,098 Da
• NCBI Official Full Name: baculoviral IAP repeat-containing protein 2 isoform 1
• NCBI Official Synonym Full Names: baculoviral IAP repeat containing 2
• NCBI Official Symbol: BIRC2
• NCBI Official Synonym Symbols: API1; MIHB; HIAP2; RNF48; cIAP1; Hiap-2; c-IAP1
• NCBI Protein Information: baculoviral IAP repeat-containing protein 2; IAP-2; IAP homolog B; apoptosis inhibitor 1; RING finger protein 48; inhibitor of apoptosis protein 2; baculoviral IAP repeat-containing 2; NFR2-TRAF signalling complex protein; TNFR2-TRAF-signaling complex protein 2
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 2
• Protein Family: Baculoviral IAP repeat-containing protein
• UniProt Gene Name: BIRC2
• UniProt Synonym Gene Names: API1; IAP2; MIHB; RNF48; IAP-2; hIAP-2; hIAP2
• UniProt Entry Name: BIRC2_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

BIRC2: Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling, and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin- protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin- protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF2, DIABLO/SMAC, MAP3K14/NIK, MAP3K5/ASK1, IKBKG/NEMO and MXD1/MAD1. Can also function as an E3 ubiquitin- protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Can stimulate the transcriptional activity of E2F1. Plays a role in the modulation of the cell cycle. Interacts with DIABLO/SMAC and with PRSS25; these interactions inhibit apoptotic suppressor activity. Interacts with CASP9. Interacts (via BIR domains) with TRAF2. Interacts with E2F1, RIPK1, RIPK2, RIPK3, RIPK4, BIRC5/survivin and USP19. Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes. The CARD domain inhibits the activation of E3 ubiquitin ligase activity by preventing RING domain dimerization and E2 ubiquitin donor binding and activation. The CARD domain- mediated autoinhibition of the E3 ubiquitin-protein ligase activity suppresses cell proliferation and migration. USP19 regulates the stability of BIRC2/c-IAP1 by preventing its ubiquitination. Belongs to the IAP family.

Protein type: Ligase; Ubiquitin conjugating system; EC 6.3.2.-; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 11q22

Cellular Component: XY body; internal side of plasma membrane; spindle microtubule; cytoplasm; cytosol; nucleus; lipid raft

Molecular Function: protein binding; zinc ion binding; transcription coactivator activity; caspase inhibitor activity; ubiquitin-protein ligase activity; protein N-terminus binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; regulation of toll-like receptor signaling pathway; response to cAMP; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein polyubiquitination; transcription, DNA-dependent; apoptosis; regulation of cell cycle; protein heterooligomerization; MyD88-independent toll-like receptor signaling pathway; toll-like receptor 3 signaling pathway; regulation of innate immune response; regulation of cell proliferation; regulation of apoptosis; response to ethanol; cell surface receptor linked signal transduction; regulation of transcription, DNA-dependent; regulation of cell differentiation; regulation of inflammatory response; response to hypoxia; toll-like receptor signaling pathway; innate immune response; toll-like receptor 4 signaling pathway; negative regulation of apoptosis; cell structure disassembly during apoptosis; placenta development