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SDS-PAGE

Smad5 recombinant protein

Recombinant Mouse Smad5 Protein

Gene Names
Smad5; Dwf-C; Madh5; MusMLP; AI451355; 1110051M15Rik
Purity
> 90 % as determined by SDS-PAGE
Synonyms
Smad5; Recombinant Mouse Smad5 Protein; SMAD5; Madh5; Msmad5; Smad5 recombinant protein
Ordering
For Research Use Only!
Host
Baculovirus-Insect Cells
Purity/Purification
> 90 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 20mM Tris, 500mM Nacl, 10% glycerol, pH8.0
Sequence Length
465
Application Notes
The recombinant mouse SMAD5 consists of 466 amino acids and predicts a molecular mass of 52.2 KDa. It migrates as an approximately 57 KDa band in SDS-PAGE under reducing conditions.
Predicted N Terminal
Gly
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

SDS-PAGE
Related Product Information for Smad5 recombinant protein
Background: SMAD5 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD5 is involved in the TGF-beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. It is also involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). The binding of ligands causes the oligomerization and phosphorylation of the SMAD5 protein. SMAD5 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.

Description: A DNA sequence encoding the mouse SMAD5 (P97454) (Thr2-Ser465) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52,172 Da
NCBI Official Full Name
mothers against decapentaplegic homolog 5
NCBI Official Synonym Full Names
SMAD family member 5
NCBI Official Symbol
Smad5
NCBI Official Synonym Symbols
Dwf-C; Madh5; MusMLP; AI451355; 1110051M15Rik
NCBI Protein Information
mothers against decapentaplegic homolog 5
UniProt Protein Name
Mothers against decapentaplegic homolog 5
UniProt Gene Name
Smad5
UniProt Synonym Gene Names
Madh5; Msmad5; MAD homolog 5; Mothers against DPP homolog 5; Dwf-C; SMAD 5; Smad5; mSmad5

Uniprot Description

SMAD5: transcription factor phosphorylated and activated by bone morphogenetic protein receptor kinases. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where they regulate transcription of target genes. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 13 B1|13 30.12 cM

Cellular Component: cytoplasm; nucleoplasm; nucleus; protein complex

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: BMP signaling pathway; cardiac muscle contraction; cartilage development; erythrocyte differentiation; germ cell development; Mullerian duct regression; osteoblast fate commitment; positive regulation of osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; transforming growth factor beta receptor signaling pathway; ureteric bud development

Research Articles on Smad5

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Product Notes

The Smad5 smad5 (Catalog #AAA2546817) is a Recombinant Protein produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant mouse SMAD5 consists of 466 amino acids and predicts a molecular mass of 52.2 KDa. It migrates as an approximately 57 KDa band in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the Smad5 smad5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Smad5, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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