Goat P4HA1 Polyclonal Antibody | anti-P4HA1 antibody

Goat Polyclonal to Human P4HA1

Cat. Num. AAA242305

• Gene Names: P4HA1; P4HA
• Reactivity: Human, Monkey, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: P4HA1; Polyclonal Antibody; Goat Polyclonal to Human P4HA1; Anti-P4HA1 Antibody (Internal) IHC-plus; 4-PH alpha-1; C-P4Halpha(I); p4HA; p4HA1; Human P4HA1; anti-P4HA1 antibody

• Host: Goat
• Reactivity: Human, Monkey, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human P4HA1. This antibody is expected to recognise both reported isoforms (NP_000908.2 and NP_001017962.1).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 534

• Applicable Applications for anti-P4HA1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: Immunohistochemistry: It was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.
• Target Species: Human
• Immunogen: P4HA1 antibody was raised against synthetic peptide C-DFARKDEPDAFKE from an internal region of human P4HA1 (NP_000908.2; NP_001017962.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Lizard (100%); Mouse, Rat, Hamster, Opossum, Salmon, Stickleback, Pufferfish, Zebrafish (92%).
• Immunogen Type: Synthetic peptide
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-P4HA1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-P4HA1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Antibody (0.2 ug/ml) staining of HEK293 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 63252886
• NCBI GeneID: 5033
• NCBI Accession #: NP_000908.2
• NCBI GenBank Nucleotide #: NM_000917.3
• UniProt Accession #: P13674; Q15082; Q15083; Q5VSQ5; C9JL12
• Molecular Weight: 58,958 Da
• NCBI Official Full Name: prolyl 4-hydroxylase subunit alpha-1 isoform 1
• NCBI Official Synonym Full Names: prolyl 4-hydroxylase, alpha polypeptide I
• NCBI Official Symbol: P4HA1
• NCBI Official Synonym Symbols: P4HA
• NCBI Protein Information: prolyl 4-hydroxylase subunit alpha-1; C-P4Halpha(I); collagen prolyl 4-hydroxylase alpha(I); procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I
• UniProt Protein Name: Prolyl 4-hydroxylase subunit alpha-1
• Protein Family: Prolyl 4-hydroxylase
• UniProt Gene Name: P4HA1
• UniProt Synonym Gene Names: P4HA; 4-PH alpha-1
• UniProt Entry Name: P4HA1_HUMAN

NCBI Description

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

P4HA1: Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. Belongs to the P4HA family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Oxidoreductase; EC 1.14.11.2; Endoplasmic reticulum; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: intracellular membrane-bound organelle; membrane; mitochondrion; endoplasmic reticulum; endoplasmic reticulum lumen

Molecular Function: oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; protein binding; procollagen-proline 4-dioxygenase activity; L-ascorbic acid binding; iron ion binding

Biological Process: collagen fibril organization; peptidyl-proline hydroxylation to 4-hydroxy-L-proline