Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Calcineurin subunit B type 1 (PPP3R1) Standard Curve.)
Rat Calcineurin Subunit B Type 1 (PPP3R1) ELISA Kit | CALNB1 elisa kit
Rat Calcineurin Subunit B Type 1 (PPP3R1) ELISA Kit
Reactivity
Rat
Synonyms
Calcineurin Subunit B Type 1 (PPP3R1); Rat Calcineurin Subunit B Type 1 (PPP3R1) ELISA Kit; PPP3R1; CNB; CNB1; calcineurin B; type I (19kDa); protein phosphatase 3 (formerly 2B); regulatory subunit B (19kD); alpha isoform (calcineurin B; type I); regulato; CALNB1 elisa kit
Reactivity
Rat
Specificity
Rat Calcineurin subunit B type 1 (PPP3R1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PPP3R1. No significant cross-reactivity or interference between Rat PPP3R1 and analogues was observed.
Sequence Length
170
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Calcineurin subunit B type 1 (PPP3R1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Calcineurin subunit B type 1 (PPP3R1) Standard Curve.)
Related Product Information for CALNB1 elisa kit
Description: This Rat Calcineurin subunit B type 1 (PPP3R1) ELISA Kit employs a two-site sandwich ELISA to quantitate PPP3R1 in samples. An antibody specific for PPP3R1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PPP3R1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPP3R1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPP3R1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Calcineurin, a calmodulin-regulated protein phosphatase, is found in the cells of all eukaryotes ranging from yeast to mammals. Wang et al. (1996) described this heterodimeric protein as having a 19-kD Ca(2+)-binding regulatory subunit, calcineurin B, and a 58- to 59-kD catalytic subunit, calcineurin A. One gene encodes calcineurin B in all tissues except testis, and it is highly conserved at the level of both protein and DNA sequences in eukaryotes. In contrast, there are 2 major isoforms, alpha and beta, of calcineurin A encoded by separate genes located on different human chromosomes. A third isoform, A-gamma, is unique to testis. Additional diversity of calcineurin A is created by alternative splicing of mRNAs. Calcineurin is especially abundant in brain where it constitutes 1% of total protein.
Background: Calcineurin, a calmodulin-regulated protein phosphatase, is found in the cells of all eukaryotes ranging from yeast to mammals. Wang et al. (1996) described this heterodimeric protein as having a 19-kD Ca(2+)-binding regulatory subunit, calcineurin B, and a 58- to 59-kD catalytic subunit, calcineurin A. One gene encodes calcineurin B in all tissues except testis, and it is highly conserved at the level of both protein and DNA sequences in eukaryotes. In contrast, there are 2 major isoforms, alpha and beta, of calcineurin A encoded by separate genes located on different human chromosomes. A third isoform, A-gamma, is unique to testis. Additional diversity of calcineurin A is created by alternative splicing of mRNAs. Calcineurin is especially abundant in brain where it constitutes 1% of total protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
calcineurin subunit B type 1
NCBI Official Synonym Full Names
protein phosphatase 3, regulatory subunit B, alpha
NCBI Official Symbol
Ppp3r1
NCBI Protein Information
calcineurin subunit B type 1
UniProt Protein Name
Calcineurin subunit B type 1
UniProt Gene Name
Ppp3r1
UniProt Synonym Gene Names
Cna2; Cnb
UniProt Entry Name
CANB1_RAT
NCBI Description
regulatory subunit of the enzyme that catalyzes the calcium-dependent removal of serine- and threonine-bound phosphate groups [RGD, Feb 2006]
Uniprot Description
PPP3R1: Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity. Belongs to the calcineurin regulatory subunit family.
Protein type: Apoptosis; Cell development/differentiation; Calcium-binding; Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis
Cellular Component: calcineurin complex; cytosol; sarcolemma
Molecular Function: calcium ion binding; enzyme binding; phosphoprotein phosphatase activity; protein binding; protein domain specific binding
Biological Process: calcineurin-NFAT signaling pathway; epithelial to mesenchymal transition; heart development; myelination in the peripheral nervous system; NFAT protein import into nucleus; patterning of blood vessels; positive regulation of NFAT protein import into nucleus; positive regulation of transcription from RNA polymerase II promoter; protein amino acid dephosphorylation; Schwann cell development
Research Articles on CALNB1
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Product Notes
The Rat CALNB1 ppp3r1 (Catalog #AAA9712096) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712096 ELISA Kit recognizes Rat CALNB1. It is sometimes possible for the material contained within the vial of "Calcineurin Subunit B Type 1 (PPP3R1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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