Smad3 recombinant protein

Recombinant Mouse Smad3 Protein (His & GST tag)

Cat. Num. AAA2546705

• Gene Names: Smad3; mad3; Madh3; Smad 3
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: Smad3; Recombinant Mouse Smad3 Protein (His & GST tag); SMAD3; Madh3; Smad3 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 2mM GSH, 10% glycerol, pH 7.4
• Sequence Length: 425

• Application Notes: The recombinant mouse SMAD3/GST chimera consists of 662 amino acids and has a calculated molecular mass of 75.9 kDa. The recombinant protein migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for Smad3 recombinant protein

Background: SMAD3 belongs to the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD3 is involved in cell signalling. It modulates signals of activin and TGFbeta's. Binding of SMAD3 with SMAD4 enables its transmigration into the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD3 is a receptor-regulated SMAD (R-SMAD). In mice, mutation of SMAD3 has been linked to colorectal adenocarcinoma, increased systemic inflammation, and accelerated wound healing. Increased SMAD3 activity has been implicated in the pathogenesis of scleroderma. Smad3 is also a multifaceted regulator in adipose physiology and the pathogenesis of obesity and type 2 diabetes.

Description: A DNA sequence encoding the mouse SMAD3 (P84025) (Met1-Ser425) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 6981174
• NCBI GeneID: 25631
• NCBI Accession #: NP_037227.1
• NCBI GenBank Nucleotide #: NM_013095.3
• UniProt Accession #: P84025; O09064; O09144; O14510; O35273; Q92940; Q93002; Q9GKR4
• Molecular Weight: 48,081 Da
• NCBI Official Full Name: mothers against decapentaplegic homolog 3
• NCBI Official Synonym Full Names: SMAD family member 3
• NCBI Official Symbol: Smad3
• NCBI Official Synonym Symbols: mad3; Madh3; Smad 3
• NCBI Protein Information: mothers against decapentaplegic homolog 3
• UniProt Protein Name: Mothers against decapentaplegic homolog 3
• Protein Family: Mothers against decapentaplegic
• UniProt Gene Name: Smad3
• UniProt Synonym Gene Names: Madh3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; SMAD 3; Smad3

NCBI Description

transcription factor; important component of the TGF-beta signaling pathway [RGD, Feb 2006]

Uniprot Description

SMAD3: transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.

Protein type: DNA-binding; Nuclear receptor co-regulator; Transcription factor

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: cytoplasm; nuclear chromatin; nuclear inner membrane; nucleoplasm; nucleus; plasma membrane; protein complex; receptor complex; transcription factor complex

Molecular Function: beta-catenin binding; bHLH transcription factor binding; chromatin binding; chromatin DNA binding; collagen binding; DNA binding; double-stranded DNA binding; enzyme binding; identical protein binding; phosphatase binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; sequence-specific DNA binding; SMAD binding; transcription factor activity; transcription factor binding; transforming growth factor beta receptor binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; ubiquitin binding; ubiquitin protein ligase binding; zinc ion binding

Biological Process: activin receptor signaling pathway; caspase activation; cell cycle arrest; developmental growth; embryonic cranial skeleton morphogenesis; embryonic foregut morphogenesis; embryonic pattern specification; endoderm development; evasion of host defenses by virus; gastrulation; heart looping; immune response; immune system development; in utero embryonic development; intercellular junction assembly and maintenance; liver development; mesoderm formation; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of fat cell differentiation; negative regulation of inflammatory response; negative regulation of mitotic cell cycle; negative regulation of osteoblast differentiation; negative regulation of osteoblast proliferation; negative regulation of protein catabolic process; negative regulation of transcription from RNA polymerase II promoter; osteoblast development; osteoblast differentiation; paraxial mesoderm morphogenesis; pericardium development; positive regulation of bone mineralization; positive regulation of cell migration; positive regulation of chondrocyte differentiation; positive regulation of focal adhesion formation; positive regulation of interleukin-1 beta production; positive regulation of nitric oxide biosynthetic process; positive regulation of positive chemotaxis; positive regulation of stress fiber formation; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor-beta3 production; protein stabilization; regulation of binding; regulation of epithelial cell proliferation; regulation of immune response; regulation of striated muscle development; regulation of transcription from RNA polymerase II promoter; regulation of transforming growth factor beta receptor signaling pathway; regulation of transforming growth factor-beta2 production; response to hypoxia; skeletal development; SMAD protein complex assembly; somitogenesis; T cell activation; thyroid gland development; transforming growth factor beta receptor signaling pathway; transport; ureteric bud development; Wnt receptor signaling pathway through beta-catenin