IL-19 recombinant protein

Recombinant human IL-19 protein

Cat. Num. AAA201337

• Gene Names: IL19; MDA1; NG.1; ZMDA1; IL-10C
• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: IL-19; Recombinant human IL-19 protein; Interleukin-19; Melanoma differentiation-associated protein-like protein; NG.1; ZMDA1; MDA1; IL-10C; IL19; Interleukin19; IL-19 recombinant protein

• Host: HEK293
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.5mg/ml (determined by absorbance at 280nm) (varies by lot)
• Sequence Positions: 25-177aa
• Sequence: LRRCL ISTDMHHIEE SFQEIKRAIQ AKDTFPNVTI LSTLETLQII KPLDVCCVTK NLLAFYVDRV FKDHQEPNPK ILRKISSIAN SFLYMQKTLR QCQEQRQCHC RQEATNATRV IHDNYDQLEV HAAAIKSLGE LDVFLAWINK NHEVMFSA

• Applicable Applications for IL-19 recombinant protein: SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: His-Tag
• Predicted N-term: Asp
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for IL-19 recombinant protein

IL-19, also known as Interleukin-19, is a member of the IL-10 family of related cytokines, which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. This protein is an immunosuppressive cytokine that is responsible for both cell type-specific and system-specific down-regulation of inflammation, because it up-regulates IL-6 and TNF-alpha, and induces apoptosis through TNF-alpha. It binds a receptor complex consisting of the IL-20 receptor alpha (IL-20 R1) and the IL-20 receptor beta (IL-20 R2). This receptor complex is also shared by IL-20 and IL-24. IL-19-deficient animals was associated with reduced numbers of IL-6-producing macrophages in the inflamed colonic lamina propria. It may contribute to the pathogenesis of inflammatory bowel disease. Recombinant human IL-19, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for IL-19 recombinant protein

Immunology; Hormones & Cytokines; Proteins

References

Gallagher, G. et al. (2000) Genes Immun. 1:442-450; Gallagher, G. et al. (2004) Int. Immunopharmacol. 4:615-626. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929-979. Dumoutier, L. et al. (2001) J. Immunol. 167:3545-3549. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517-47523. Jordan, W.J. et al. (2005) Eur. J. Immunol. 35:1576-1582.

NCBI and Uniprot Product Information

• NCBI GI #: 30795208
• NCBI GeneID: 29949
• NCBI Accession #: NP_037503.2
• NCBI GenBank Nucleotide #: NM_013371.3
• UniProt Accession #: Q9UHD0; Q5VUT3; Q96QR4; Q9NUA0; B6VEV9
• Molecular Weight: 23,079 Da
• NCBI Official Full Name: interleukin-19 isoform 2
• NCBI Official Synonym Full Names: interleukin 19
• NCBI Official Symbol: IL19
• NCBI Official Synonym Symbols: MDA1; NG.1; ZMDA1; IL-10C
• NCBI Protein Information: interleukin-19; melanoma differentiation associated protein-like protein; melanoma differentiation-associated protein-like protein
• UniProt Protein Name: Interleukin-19
• UniProt Gene Name: IL19
• UniProt Synonym Gene Names: ZMDA1; IL-19
• UniProt Entry Name: IL19_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the IL10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Alternatively spliced transcript variants encoding the distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

IL19: May play some important roles in inflammatory responses. Up-regulates IL-6 and TNF-alpha and induces apoptosis. Belongs to the IL-10 family.

Protein type: Secreted; Apoptosis; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32.2

Cellular Component: extracellular space; extracellular region

Molecular Function: cytokine activity

Biological Process: apoptosis; positive regulation of JAK-STAT cascade; immune response; inflammatory response; signal transduction; interleukin-6 biosynthetic process