Human solute carrier family 30 (zinc transporter) , member 8 ELISA Kit | SLC30A8 elisa kit

Human Zinc transporter 8, SLC30A8 ELISA Kit

Cat. Num. AAA947677

• Gene Names: SLC30A8; ZNT8; ZnT-8
• Reactivity: Human
• Synonyms: solute carrier family 30 (zinc transporter); member 8; Human Zinc transporter 8; SLC30A8 ELISA Kit; Human Zinc transporter 8 (SLC30A8) ELISA kit; ZNT8; ZnT-8; solute carrier family 30 member 8; zinc transporter ZnT-8; SLC30A8 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human SLC30A8. No significant cross-reactivity or interference between human SLC30A8 and analogues was observed.
• Sequence Length: 320

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 80 pg/ml -1400 pg/ml.
• Sensitivity: The minimum detectable dose of human SLC30A8 is typically less than 50 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human SLC30A8 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SLC30A8 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SLC30A8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for SLC30A8. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC30A8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 289803003
• NCBI GeneID: 169026
• NCBI Accession #: NP_001166282.1
• NCBI GenBank Nucleotide #: NM_001172811.1
• UniProt Accession #: Q8IWU4; Q8TCL3; A0AVP9; A5YM39; B4DPE0
• Molecular Weight: 40,755 Da
• NCBI Official Full Name: zinc transporter 8 isoform b
• NCBI Official Synonym Full Names: solute carrier family 30 (zinc transporter), member 8
• NCBI Official Symbol: SLC30A8
• NCBI Official Synonym Symbols: ZNT8; ZnT-8
• NCBI Protein Information: zinc transporter 8; zinc transporter ZnT-8
• UniProt Protein Name: Zinc transporter 8
• Protein Family: Zinc transporter
• UniProt Gene Name: SLC30A8
• UniProt Synonym Gene Names: ZNT8; ZnT-8
• UniProt Entry Name: ZNT8_HUMAN

NCBI Description

The protein encoded by this gene is a zinc efflux transporter involved in the accumulation of zinc in intracellular vesicles. This gene is expressed at a high level only in the pancreas, particularly in islets of Langerhans. The encoded protein colocalizes with insulin in the secretory pathway granules of the insulin-secreting INS-1 cells. Allelic variants of this gene exist that confer susceptibility to diabetes mellitus, noninsulin-dependent (NIDDM). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]

Uniprot Description

SLC30A8: Facilitates the accumulation of zinc from the cytoplasm into intracellular vesicles, being a zinc-efflux transporter. May be a major component for providing zinc to insulin maturation and/or storage processes in insulin-secreting pancreatic beta- cells. Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 8q24.11

Cellular Component: Golgi apparatus; secretory granule membrane; cytoplasmic membrane-bound vesicle; integral to membrane; plasma membrane; secretory granule

Molecular Function: protein homodimerization activity; zinc ion binding; zinc ion transmembrane transporter activity

Biological Process: sequestering of zinc ion; cellular protein metabolic process; insulin secretion; cellular zinc ion homeostasis; response to glucose stimulus; positive regulation of insulin secretion; zinc ion transport; glucose homeostasis; transmembrane transport

Disease: Diabetes Mellitus, Noninsulin-dependent