<
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A:NIH-3T3) using a 7.5 % SDS PAGE gel and FAST antibody at a dilution of 1:1000)
Rabbit FAST Polyclonal Antibody | anti-FAST antibody
FAST antibody
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
FAST antibody was purified by Protein A chromatography
Synonyms
FAST; Polyclonal Antibody; FAST antibody; Polyclonal FAST; Anti-FAST; FASTK; fas-activated serine/threonine kinase; FAST kinase; anti-FAST antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
FAST antibody was purified by Protein A chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
6.56 mg/ml (varies by lot)
Applicable Applications for anti-FAST antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF:1:100-1:500
ICC:1:100-1:500
IHC-P: 1:100-1:500
WB: 1:500-1:3000
ICC:1:100-1:500
IHC-P: 1:100-1:500
WB: 1:500-1:3000
Biological Significance
The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ligation, it phosphorylates TIA1, an apoptosis-promoting nuclear RNA-binding protein. The encoded protein is a strong inducer of lymphocyte apoptosis.
Cross-Reactivity
Human, Mouse
Immunogen
FAST antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 346 and 549 of FAST
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A:NIH-3T3) using a 7.5 % SDS PAGE gel and FAST antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A:NIH-3T3) using a 7.5 % SDS PAGE gel and FAST antibody at a dilution of 1:1000)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded SCC15 xenograft using FAST antibody at a dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded SCC15 xenograft using FAST antibody at a dilution of 1:100)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: MOLT4; B: Raji) using a 12 % SDS PAGE gel and FAST antibody at a dilution of 1:500)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: MOLT4; B: Raji) using a 12 % SDS PAGE gel and FAST antibody at a dilution of 1:500)
Immunofluorescence (IF)
(Immunofluorescence analysis of methanol-fixed HeLa, using FAST antibody at 1:200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of methanol-fixed HeLa, using FAST antibody at 1:200 dilution.)
Related Product Information for anti-FAST antibody
Affinity purified Rabbit polyclonal FAST antibody
Product Categories/Family for anti-FAST antibody
Similar Products
Product Notes
The FAST (Catalog #AAA835264) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's FAST can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF:1:100-1:500 ICC:1:100-1:500 IHC-P: 1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the FAST for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAST, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
