Mouse Secondary Lymphoid Tissue Chemokine ELISA Kit | SLC elisa kit

Mouse Secondary Lymphoid Tissue Chemokine ELISA Kit

Cat. Num. AAA762220

• Gene Names: CCL21; ECL; SLC; CKb9; TCA4; 6Ckine; SCYA21
• Reactivity: Mouse
• Synonyms: Secondary Lymphoid Tissue Chemokine; Mouse Secondary Lymphoid Tissue Chemokine ELISA Kit; SLC/CCL21/6Ckine/CKb9/ECL/SCYA21/TCA4; SLC elisa kit

• Reactivity: Mouse
• Sequence Length: 134

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for SLC elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse SLC antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse SLC antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse SLC amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse SLC can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4128129
• NCBI GeneID: 6366
• UniProt Accession #: O00585
• Molecular Weight: 14,646 Da
• NCBI Official Full Name: secondary lymphoid tissue chemokine
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 21
• NCBI Official Symbol: CCL21
• NCBI Official Synonym Symbols: ECL; SLC; CKb9; TCA4; 6Ckine; SCYA21
• NCBI Protein Information: C-C motif chemokine 21
• UniProt Protein Name: C-C motif chemokine 21
• UniProt Gene Name: CCL21
• UniProt Synonym Gene Names: SCYA21; SLC
• UniProt Entry Name: CCL21_HUMAN

NCBI Description

This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19). [provided by RefSeq, Sep 2014]

Uniprot Description

CCL21: Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Chemokine

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: extracellular region; extracellular space; intracellular

Molecular Function: CCR chemokine receptor binding; CCR7 chemokine receptor binding; chemokine activity; chemokine receptor binding

Biological Process: cell maturation; cell-cell signaling; dendritic cell chemotaxis; establishment of T cell polarity; formation of immunological synapse; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; lymphocyte chemotaxis; monocyte chemotaxis; neutrophil chemotaxis; positive regulation of actin filament polymerization; positive regulation of cell adhesion mediated by integrin; positive regulation of cell-matrix adhesion; positive regulation of chemotaxis; positive regulation of dendritic cell antigen processing and presentation; positive regulation of filopodium formation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of JNK cascade; positive regulation of phosphoinositide 3-kinase activity; positive regulation of protein kinase activity; positive regulation of protein kinase B signaling cascade; positive regulation of pseudopodium formation; positive regulation of receptor-mediated endocytosis; release of sequestered calcium ion into cytosol; ruffle organization and biogenesis; T cell costimulation