Western Blot (WB)
(Western blot analysis of extracts of 293T cells, using NEK6 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Rabbit anti-Human NEK6 Polyclonal Antibody | anti-NEK6 antibody
NEK6 Polyclonal Antibody
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of 293T cells, using NEK6 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of 293T cells, using NEK6 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
NEK6: a protein kinase of the NEK family. Important in mitosis. May regulate spindle function.
Protein type: Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Other group; NEK family
Chromosomal Location of Human Ortholog: 9q33.3-q34.11
Cellular Component: spindle pole; microtubule; cytoplasm; microtubule organizing center; nuclear speck; cytosol; nucleus
Molecular Function: protein serine/threonine kinase activity; signal transducer activity; protein binding; kinesin binding; magnesium ion binding; protein kinase binding; ATP binding
Biological Process: mitosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; protein amino acid autophosphorylation; regulation of mitotic cell cycle; regulation of mitotic metaphase/anaphase transition; mitotic nuclear envelope disassembly; cytokinesis; spindle assembly; signal transduction; protein amino acid phosphorylation; chromosome segregation; peptidyl-serine phosphorylation; mitotic cell cycle; G2/M transition DNA damage checkpoint
Research Articles on NEK6
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Product Notes
The NEK6 nek6 (Catalog #AAA9128050) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NEK6 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NEK6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB 1:500 - 1:2000. Researchers should empirically determine the suitability of the NEK6 nek6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEK6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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