SIGLEC5 recombinant protein

SIGLEC5 Protein, Human, Recombinant

Cat. Num. AAA8120975

• Gene Names: SIGLEC5; CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
• Purity: >85% as determined by SDS-PAGE
• Synonyms: SIGLEC5; SIGLEC5 Protein; Human; Recombinant; Human SIGLEC5 Protein; CD170 Protein; CD33L2 Protein; OB-BP2 Protein; OBBP Protein; OBBP2 Protein; SIGLEC-5 Protein; sialic acid binding Ig like lectin 5; SIGLEC5 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr434

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 17
• Tag: C-cleavage
• Protein Construction: A DNA sequence encoding the human SIGLEC5 (O15389)(Met1-Thr 434) was expressed with six amino acids (LEVLFQ) at the C-terminus was expressed and purified.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SIGLEC5 recombinant protein

Background: SIGLEC5 contains 2 Ig-like C2-type (immunoglobulin-like) domains and 1 Ig-like V-type (immunoglobulin-like) domain. It belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. SIGLEC5 is expressed by monocytic/myeloid lineage cells. It is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. It is also expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation. SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Product Categories/Family for SIGLEC5 recombinant protein

ITIM/ITAM Immunoreceptors and Related Molecules

References

Angata T, et al. (2006) Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates. FASEB J. 20(12):1964-73.Avril T, et al. (2005) Siglec-5 (CD170) can mediate inhibitory signaling in the absence of immunoreceptor tyrosine-based inhibitory motif phosphorylation. J Biol Chem. 280(20):19843-51.Erickson-Miller CL, et al. (2003) Characterization of Siglec-5 (CD170) expression and functional activity of anti-Siglec-5 antibodies on human phagocytes. Exp Hematol. 31(5):382-8.

NCBI and Uniprot Product Information

• NCBI GI #: 25009254
• NCBI GeneID: 8778
• UniProt Accession #: O15389
• Molecular Weight: 60,715 Da
• NCBI Official Full Name: Sialic acid-binding Ig-like lectin 5
• NCBI Official Synonym Full Names: sialic acid binding Ig like lectin 5
• NCBI Official Symbol: SIGLEC5
• NCBI Official Synonym Symbols: CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
• NCBI Protein Information: sialic acid-binding Ig-like lectin 5
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 5
• Protein Family: Sialic acid-binding Ig-like lectin
• UniProt Gene Name: SIGLEC5
• UniProt Synonym Gene Names: CD33L2; OBBP2; Siglec-5; OB-BP2; OB-binding protein 2

NCBI Description

This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion. [provided by RefSeq, Feb 2012]

Uniprot Description

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.