Human Eukaryotic Translation Initiation Factor 6 (EIF6) ELISA Kit | EIF6 elisa kit

Human Eukaryotic Translation Initiation Factor 6 (EIF6) ELISA Kit

Cat. Num. AAA2706940

• Gene Names: Eif6; CAB; eIF-6; p27BBP; Itgb4bp; imc-415; AA408895; p27(BBP); 1110004P11
• Reactivity: Human
• Synonyms: Eukaryotic Translation Initiation Factor 6 (EIF6); Human Eukaryotic Translation Initiation Factor 6 (EIF6) ELISA Kit; ITGB4BP; CAB; p27BBP; Integrin Beta 4 Binding Protein; B4 integrin interactor; EIF6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Eukaryotic Translation Initiation Factor 6 (EIF6).; No significant cross-reactivity or interference between Eukaryotic Translation Initiation Factor 6 (EIF6) and analogues was observed.
• Sequence Length: 245

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Eukaryotic Translation Initiation Factor 6 (EIF6) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Eukaryotic Translation Initiation Factor 6 (EIF6) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EIF6 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eukaryotic Translation Initiation Factor 6 (EIF6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eukaryotic Translation Initiation Factor 6 (EIF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eukaryotic Translation Initiation Factor 6 (EIF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Eukaryotic Translation Initiation Factor 6 (EIF6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 27501448
• NCBI GeneID: 16418
• NCBI Accession #: NP_034709.1
• NCBI GenBank Nucleotide #: NM_010579.2
• UniProt Accession #: O55135
• Molecular Weight: 26,511 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 6
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 6
• NCBI Official Symbol: Eif6
• NCBI Official Synonym Symbols: CAB; eIF-6; p27BBP; Itgb4bp; imc-415; AA408895; p27(BBP); 1110004P11
• NCBI Protein Information: eukaryotic translation initiation factor 6
• UniProt Protein Name: Eukaryotic translation initiation factor 6
• Protein Family: Translation initiation factor
• UniProt Gene Name: Eif6
• UniProt Synonym Gene Names: Itgb4bp; eIF-6

NCBI Description

The protein encoded by this gene is a eukaryotic translation initiation factor that regulates both ribosome biogenesis and translation, which are rate-limiting factors for cell growth. The encoded protein binds 60S ribosomes and prevents their association with 40S ribosomes. This gene may play a role in oncogenesis as well. [provided by RefSeq, Aug 2015]

Uniprot Description

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity. In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR (PubMed:26383020). Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis (PubMed:26391622). Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (). Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth (PubMed:21665150).