Rabbit ABCA5 Polyclonal Antibody | anti-ABCA5 antibody

ABCA5 Polyclonal Antibody

Cat. Num. AAA3006385

• Gene Names: ABCA5; HTC3; ABC13; EST90625
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ABCA5; Polyclonal Antibody; ABCA5 Polyclonal Antibody; ATP-binding cassette sub-family A member 5; KIAA1888; anti-ABCA5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ABCA5 polyclonal antibody detects endogenous levels of ABCA5 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1642

• Applicable Applications for anti-ABCA5 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human ABCA5.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ABCA5 polyclonal antibody at 1:500 dilutionLane1:HepG2 whole cell lysateLane2:NIH-3T3 whole cell lysateLane3:H9C2 whole cell lysate)

Related Product Information for anti-ABCA5 antibody

ABCA5 (ATP-binding cassette, sub-family A (ABC1), member 5) is a 1, 642 amino acid protein belonging to the ABC transporter superfamily and the ABCA family. The ABC1 subfamily is the only major ABC subfamily exclusive to multicellular eukaryotes. Ubiquitously expressed, with high expression in testis, skeletal muscle, kidney, liver and placenta, ABCA5 is a multi-pass membrane protein that contains two ABC transporter domains and exists as three alternatively spliced isoforms. ABCA5 exhibits membrane subcellular localization and may play a role in processing autolysosomes. Spanning 80 kb, ABCA5 contains 39 exons, with exon 2 containing the putative translation start site. ABCA5 is the first of five ABC1 family members that maps to human chromosome 17q24. ABCA5 is linked to lysosomal diseases and may play a role in tumor development and cardiomyocyte and follicular cell activities. ABCA5 might also be a specific urine marker for test of patients with high-grade prostatic intraepithelial neoplasia (HGPIN).

NCBI and Uniprot Product Information

• NCBI GI #: 27262624
• NCBI GeneID: 23461
• NCBI Accession #: NP_061142.2
• NCBI GenBank Nucleotide #: NP_061142.2
• UniProt Accession #: Q8WWZ7
• Molecular Weight: ~ 186kDa
• NCBI Official Full Name: ATP-binding cassette sub-family A member 5
• NCBI Official Synonym Full Names: ATP binding cassette subfamily A member 5
• NCBI Official Symbol: ABCA5
• NCBI Official Synonym Symbols: HTC3; ABC13; EST90625
• NCBI Protein Information: ATP-binding cassette sub-family A member 5
• UniProt Protein Name: ATP-binding cassette sub-family A member 5
• Protein Family: ABC transporter A family
• UniProt Gene Name: ABCA5
• UniProt Synonym Gene Names: KIAA1888
• UniProt Entry Name: ABCA5_HUMAN

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White). This encoded protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This gene is clustered among 4 other ABC1 family members on 17q24, but neither the substrate nor the function of this gene is known. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCA5: May play a role in the processing of autolysosomes. Belongs to the ABC transporter superfamily. ABCA family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter; Membrane protein, integral; Transporter, ABC family

Chromosomal Location of Human Ortholog: 17q24.3

Cellular Component: Golgi membrane; late endosome membrane; lysosomal membrane; lysosome; late endosome; integral to membrane

Molecular Function: transporter activity; ATPase activity; ATP binding

Biological Process: reverse cholesterol transport; metabolic process; cholesterol efflux