Typical Testing Data/Standard Curve (for reference only)
IL-6 elisa kit
Murine IL-6 ELISA Kit
Gene Names
Il6; ILg6; Ifnb2
Reactivity
Murine. No cross reactivity with other murine cytokines.
Synonyms
IL-6; Murine IL-6 ELISA Kit; IL-6 elisa kit
Reactivity
Murine. No cross reactivity with other murine cytokines.
Sequence Length
211
Samples
Supernatants, buffered solutions or serum and plasma samples. this assay will recogni??e both natural and recombinant murine il-6
Assay Type
Quantitative Sandwich
Sensitivity
< 10 pg/ml
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL-6 elisa kit
Intended Uses: The murine IL-6 ELISA kit is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of murine Interleukin-6 in supernatants, buffered solutions or serum and plasma samples. This assay will recogni??e both natural and recombinant murine IL-6.
Principle of the Assay: A capture Antibody highly specific for murine IL-6 has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of murine IL-6 samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti-murine IL-6 secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of murine IL-6 present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of murine IL-6 in any sample tested.
Principle of the Assay: A capture Antibody highly specific for murine IL-6 has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of murine IL-6 samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti-murine IL-6 secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of murine IL-6 present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of murine IL-6 in any sample tested.
Product Categories/Family for IL-6 elisa kit
References
Garcia-Perez, M. A. et al., Hum Reprod.,2006; 21(4): 880-7
Gironella, M. et al., J Leukoc Biol.,2002;72(1): 56-64.
Izawa, A. et al. Infect. Immun., 2014 ; 82(5): 1904-1913
Gironella, M. et al., J Leukoc Biol.,2002;72(1): 56-64.
Izawa, A. et al. Infect. Immun., 2014 ; 82(5): 1904-1913
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,357 Da
NCBI Official Full Name
interleukin-6
NCBI Official Synonym Full Names
interleukin 6
NCBI Official Symbol
Il6
NCBI Official Synonym Symbols
ILg6; Ifnb2
NCBI Protein Information
interleukin-6
UniProt Protein Name
Interleukin-6
UniProt Gene Name
Il6
UniProt Synonym Gene Names
Il-6; IL-6
UniProt Entry Name
IL6_RAT
NCBI Description
a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]
Uniprot Description
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation ().
Research Articles on IL-6
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Product Notes
The Murine. No cross reactivity with other murine cytokines. IL-6 il6 (Catalog #AAA335514) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA335514 ELISA Kit recognizes Murine. No cross reactivity with other murine cytokines. IL-6. It is sometimes possible for the material contained within the vial of "IL-6, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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