Rat sex hormone-binding globulin ELISA Kit | SHBG elisa kit

Rat sex hormone-binding globulin, SHBG ELISA Kit

Cat. Num. AAA902439

• Reactivity: Rat
• Synonyms: sex hormone-binding globulin; Rat sex hormone-binding globulin; SHBG ELISA Kit; Rat sex hormone-binding globulin (SHBG) ELISA kit; ABP; MGC126834; MGC138391; SBP; TEBG; androgen binding protein; sex steroid-binding protein; testis-specific androgen-binding protein; testosterone-binding beta-globulin; testosterone-estradiol-binding; SHBG elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat SHBG. No significant cross-reactivity or interference between Rat SHBG and analogues was observed.
• Sequence Length: 398

• Samples: Serum, plasma, cell culture supernates, tissue homogenates, Cell lysates
• Assay Type: Competitive
• Detection Range: 375 ng/ml-6000 ng/ml
• Sensitivity: 187.5ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SHBG elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SHBG. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated SHBG. A competitive inhibition reaction is launched between SHBG (Standards or samples) and Biotin-conjugated SHBG with the pre-coated antibody specific for SHBG. The more amount of SHBG in samples, the less antibody bound by Biotin-conjugated SHBG. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of SHBG in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 126723743
• NCBI GeneID: 100009224
• NCBI Accession #: NP_001075839.1
• NCBI GenBank Nucleotide #: NM_001082370.1
• UniProt Accession #: P15196; Q28663; Q28669
• Molecular Weight: 43,154 Da
• NCBI Official Full Name: sex hormone-binding globulin
• NCBI Official Symbol: SHBG
• NCBI Protein Information: sex hormone-binding globulin; ABP; SBP; sex steroid-binding protein; sex hormone binding globulin; androgen binding protein 1 (296 AA); sex hormone-binding globulin protein; testis-specific androgen-binding protein
• UniProt Protein Name: Sex hormone-binding globulin
• Protein Family: Sex hormone-binding globulin
• UniProt Gene Name: SHBG
• UniProt Synonym Gene Names: SHBG; SBP; ABP
• UniProt Entry Name: SHBG_RABIT

Uniprot Description

Function: Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration