Mouse sex hormone-binding globulin ELISA Kit | SHBG elisa kit

Mouse sex hormone-binding globulin, SHBG ELISA Kit

Cat. Num. AAA703219

• Gene Names: Shbg; ABP
• Reactivity: Mouse
• Synonyms: sex hormone-binding globulin; Mouse sex hormone-binding globulin; SHBG ELISA Kit; ABP; MGC126834; MGC138391; SBP; TEBG; androgen binding protein; sex steroid-binding protein; testis-specific androgen-binding protein; testosterone-binding beta-globulin; testosterone-estradiol-binding; SHBG elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse SHBG. No significant cross-reactivity or interference between Mouse SHBG and analogues was observed.
• Sequence Length: 403

• Detection Range: 1.7 ng/ml-50 ng/ml
• Sensitivity: 0.4 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SHBG elisa kit

Introduction: Sex hormone-binding globulin (SHBG) is a glycoprotein that binds to sex hormones, specifically testosterone and estradiol. Other steroid hormones such as progesterone, cortisol, and other corticosteroids are bound by transcortin. SHBG is produced by the liver cells and is released into the bloodstream. Other sites that produce SHBG are the brain, uterus, and placenta. In addition SHBG is produced by the testes; testes-produced SHBG is also called androgen-binding protein. The gene for SHBG is located on chromosome 17. SHBG levels appear to be controlled by a delicate balance of enhancing and inhibiting factors. Its level is decreased by high levels of insulin and insulin-like growth factor 1 (IGF-1). Also, high androgen and transcortin levels decrease SHBG, while high growth hormone, estrogen and thyroxine levels increase it. However, recent evidence suggests that it is the liver's production of fats that reduces SHBG levels, not any direct effect of insulin and specific genetic mechanisms have been found that do this.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SHBG antibody. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated monoclonal antibody preparation specific for SHBG and incubated. Then substrate solution A and B are added to each well. Only those wells that contain SHBG, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SHBG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6755506
• NCBI GeneID: 20415
• NCBI Accession #: NP_035497.1
• NCBI GenBank Nucleotide #: NM_011367.2
• UniProt Accession #: P97497; Q0VB52
• Molecular Weight: 44,816 Da
• NCBI Official Full Name: sex hormone-binding globulin
• NCBI Official Synonym Full Names: sex hormone binding globulin
• NCBI Official Symbol: Shbg
• NCBI Official Synonym Symbols: ABP
• NCBI Protein Information: sex hormone-binding globulin; SBP; sex steroid-binding protein; testis-specific androgen-binding protein
• UniProt Protein Name: Sex hormone-binding globulin
• Protein Family: Sex hormone-binding globulin
• UniProt Gene Name: Shbg
• UniProt Synonym Gene Names: SHBG; SBP; ABP
• UniProt Entry Name: SHBG_MOUSE

Uniprot Description

Function: Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration