SH2D1B recombinant protein

SH2D1B, 1-132aa, Human, His-tag, Baculovirus

Cat. Num. AAA206707

• Gene Names: SH2D1B; EAT2
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: SH2D1B; 1-132aa; Human; His-tag; Baculovirus; SH2 domain-containing protein 1B; EAT2; SH2D1B recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 20% glycerol.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPMDLPYYH GRLTKQDCET LLLKEGVDGN FLLRDSESIP GVLCLCVSFK NIVYTYRIFR EKHGYYRIQT AEGSPKQVFP SLKELISKFE KPNQGMVVHL LKPIKRTSPS LRWRGLKLEL ETFVNSNSDY VDVLPHHHHH H
• Sequence Length: 132

• Applicable Applications for SH2D1B recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for SH2D1B recombinant protein

SH2D1B, also known as SH2 domain-containing protein 1B, is one of the proteins which activate NK cells. It plays a role in controlling signal transduction through at least four receptors, CD84, SLAMF1, LY9 and CD244, expressed on the surface of professional antigen-presenting cells. Two isoforms of the human protein are produced by alternative splicing. Recombinant human SH2D1B protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for SH2D1B recombinant protein

Immunology; NK Cells

References

Perez-Quintero LA., et al, (2014) J Exp Med. 211:727-742.; Aldhamen YA., et al, (2012) J Immunol. 189:1349-1359.

NCBI and Uniprot Product Information

• NCBI GI #: 54792745
• NCBI GeneID: 117157
• NCBI Accession #: NP_444512
• NCBI GenBank Nucleotide #: NM_053282.4
• UniProt Accession #: O14796; Q5T0L1; Q8NI18; Q969K9; B2RBN6
• Molecular Weight: 16.4kDa (141aa) 18-28KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: SH2 domain-containing protein 1B
• NCBI Official Synonym Full Names: SH2 domain containing 1B
• NCBI Official Symbol: SH2D1B
• NCBI Official Synonym Symbols: EAT2
• NCBI Protein Information: SH2 domain-containing protein 1B
• UniProt Protein Name: SH2 domain-containing protein 1B
• Protein Family: SH2 domain-containing protein
• UniProt Gene Name: SH2D1B
• UniProt Synonym Gene Names: EAT2; EAT-2

NCBI Description

By binding phosphotyrosines through its free SRC (MIM 190090) homology-2 (SH2) domain, EAT2 regulates signal transduction through receptors expressed on the surface of antigen-presenting cells (Morra et al., 2001 [PubMed 11689425]).[supplied by OMIM, Mar 2008]

Uniprot Description

Cytoplasmic adapter regulating receptors of the signaling lymphocytic activation molecule (SLAM) family such as CD84, SLAMF1, LY9 and CD244 (PubMed:11689425). In SLAM signaling seems to cooperate with SH2D1A/SAP. Plays a role in regulation of effector functions of natural killer (NK) cells by controlling signal transduction through CD244/2B4 without effecting its tyrosine phosphorylation; downstream signaling involves PLCG1 and ERK activation (PubMed:24687958). Activation of SLAMF7-mediated NK cell function does not effect receptor tyrosine phosphorylation but distal signaling (). In the context of NK cell-mediated cytotoxicity does not enhance conjugate formation with target cells but stimulates polarization of the microtubule-organizing center and cytotoxic granules toward the NK cell synapse (PubMed:24687958). Negatively regulates CD40-induced cytokine production in dendritic cells downstream of SLAM family receptors probably by inducing activation of the PI3K pathway to inhibit p38 MAPK and JNK activation ().