Rabbit anti-Human FZD7 Polyclonal Antibody | anti-FZD7 antibody

FZD7, ID (FZD7, Frizzled-7, FzE3) (APC)

Cat. Num. AAA6300599

• Gene Names: FZD7; FzE3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FZD7; Polyclonal Antibody; ID (FZD7; Frizzled-7; FzE3) (APC); FzE3; anti-FZD7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-FZD7 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FZD7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 201-230 amino acids from the Central region of human FZD7.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(FZD7 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the FZD7 antibody detected the FZD7 protein (arrow).)

Related Product Information for anti-FZD7 antibody

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas.

Product Categories/Family for anti-FZD7 antibody

Antibodies; Wnt Proteins

References

Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010). Vincan, E., et al. Dev. Dyn. 239(1):311-317(2010). Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010): Hosgood, H.D. III, et al. Respir Med 103(12):1866-1870(2009). Ueno, K., et al. Br. J. Cancer 101(8):1374-1381(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4503833
• NCBI GeneID: 8324
• NCBI Accession #: NP_003498.1
• NCBI GenBank Nucleotide #: NM_003507.1
• UniProt Accession #: O75084; O94816; Q53S59; Q96B74
• Molecular Weight: 63,620 Da
• NCBI Official Full Name: frizzled-7
• NCBI Official Synonym Full Names: frizzled class receptor 7
• NCBI Official Symbol: FZD7
• NCBI Official Synonym Symbols: FzE3
• NCBI Protein Information: frizzled-7
• UniProt Protein Name: Frizzled-7
• Protein Family: Frizzled
• UniProt Gene Name: FZD7
• UniProt Synonym Gene Names: Fz-7; hFz7
• UniProt Entry Name: FZD7_HUMAN

NCBI Description

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. [provided by RefSeq, Jul 2008]

Uniprot Description

FZD7: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: Receptor, GPCR; Motility/polarity/chemotaxis; Membrane protein, multi-pass; GPCR, Fz/Smo family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity; protein binding; frizzled binding; PDZ domain binding

Biological Process: neuron differentiation; G-protein coupled receptor protein signaling pathway; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of epithelial cell proliferation involved in wound healing; stem cell maintenance; T cell differentiation in the thymus; positive regulation of JNK cascade; somatic stem cell division; Wnt receptor signaling pathway through beta-catenin; satellite cell compartment self-renewal involved in skeletal muscle regeneration; negative regulation of ectodermal cell fate specification; positive regulation of phosphorylation