Rabbit anti-Human PYCR1 Polyclonal Antibody | anti-PYCR1 antibody

PYCR1, CT (PYCR1, Pyrroline-5-carboxylate reductase 1, mitochondrial) (Azide free) (HRP)

Cat. Num. AAA6339331

• Gene Names: PYCR1; P5C; P5CR; PRO3; PYCR; PIG45; PP222; ARCL2B; ARCL3B
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PYCR1; Polyclonal Antibody; CT (PYCR1; Pyrroline-5-carboxylate reductase 1; mitochondrial) (Azide free) (HRP); mitochondrial; anti-PYCR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PYCR1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PYCR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290~319 amino acids from the C-terminal region of human PYCR1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PYCR1 Antibody (C-term) western blot analysis in K562,WiDr,HepG2 cell line lysates (35ug/lane).This demonstrates the PYCR1 antibody detected the PYCR1 protein (arrow).)

Immunohistochemistry (IHC)

(PYCR1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PYCR1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PYCR1 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PYCR1 antibody

This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion.

Product Categories/Family for anti-PYCR1 antibody

Antibodies; Mitochondrial Proteins

References

Reversade, B., et al. Nat. Genet. 41(9):1016-1021(2009). Guernsey, D.L., et al. Am. J. Hum. Genet. 85(1):120-129(2009). Meng, Z., et al. J. Mol. Biol. 359(5):1364-1377(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 533112488
• NCBI GeneID: 5831
• NCBI Accession #: NP_001269209.1
• NCBI GenBank Nucleotide #: NM_001282280.1
• UniProt Accession #: P32322; Q6FHI4; Q96DI6; Q9HBQ4; A6NFM2; B4DMU0
• Molecular Weight: 35,981 Da
• NCBI Official Full Name: pyrroline-5-carboxylate reductase 1, mitochondrial isoform 1
• NCBI Official Synonym Full Names: pyrroline-5-carboxylate reductase 1
• NCBI Official Symbol: PYCR1
• NCBI Official Synonym Symbols: P5C; P5CR; PRO3; PYCR; PIG45; PP222; ARCL2B; ARCL3B
• NCBI Protein Information: pyrroline-5-carboxylate reductase 1, mitochondrial; P5C reductase 1; proliferation-inducing protein 45
• UniProt Protein Name: Pyrroline-5-carboxylate reductase 1, mitochondrial
• Protein Family: Pyrroline-5-carboxylate reductase
• UniProt Gene Name: PYCR1
• UniProt Synonym Gene Names: P5C reductase 1; P5CR 1
• UniProt Entry Name: P5CR1_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

PYCR1: Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress. Defects in PYCR1 are the cause of cutis laxa autosomal recessive type 2B (ARCL2B). A multisystem disorder characterized by the appearance of premature aging, wrinkled and lax skin with reduced elasticity, joint laxity, craniofacial dysmorphic features, intrauterine growth retardation with some degree of postnatal growth deficiency, and developmental delay. Defects in PYCR1 are the cause of cutis laxa, autosomal recessive, type 3B (ARCL3B). ARCL3B is a disorder characterized by an aged appearance with distinctive facial features, sparse hair, ophthalmologic abnormalities, intrauterine growth retardation, and cutis laxa. Belongs to the pyrroline-5-carboxylate reductase family.

Protein type: EC 1.5.1.2; Mitochondrial; Oxidoreductase; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: identical protein binding; protein binding; pyrroline-5-carboxylate reductase activity

Biological Process: regulation of mitochondrial membrane potential; proline biosynthetic process; amino acid biosynthetic process

Disease: Cutis Laxa, Autosomal Recessive, Type Iib; Cutis Laxa, Autosomal Recessive, Type Iiib