sFRP-2 recombinant protein

Recombinant mouse sFRP-2 protein

Cat. Num. AAA201286

• Gene Names: Sfrp2; Sdf5; AI851596
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: sFRP-2; Recombinant mouse sFRP-2 protein; Secreted frizzled-related protein 2; sFRP2; Sfrp2; Protein SDF5; Sdf5; AI851596; sdf; SDF-5; FRP-2secreted apoptosis related protein 1; Secreted apoptosis-related protein 1; SARP-1; Sarp1; SARP1; sFRP-2 recombinant protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 100mM NaCl, 20% glycerol
• Concentration: 0.25mg/ml (determined by absorbance at 280nm) (varies by lot)
• Sequence Positions: 25-295aa
• Sequence: LFLFGQP DFSYKRSNCK PIPANLQLCH GIEYQNMRLP NLLGHETMKE VLEQAGAWIP LVMKQCHPDT KKFLCSLFAP VCLDDLDETI QPCHSLCVQV KDRCAPVMSA FGFPWPDMLE CDRFPQDNDL CIPLASSDHL LPATEEAPKV CEACKTKNED DNDIMETLCK NDFALKIKVK EITYINRDTK IILETKSKTI YKLNGVSERD LKKSVLWLKD SLQCTCEEMN DINAPYLVMG QKQGGELVIT SVKRWQKGQR EFKRISRSIR KLQC

• Applicable Applications for sFRP-2 recombinant protein: SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Mouse
• Tag: His-Tag
• Predicted N-term: Ala
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for sFRP-2 recombinant protein

sFRP-2, also known as Secreted apoptosis-related protein 1, is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. sFRP-2 can exert either positive or negative effects on Wnt signaling. At physiological concentrations, it also inhibits BMP-induced effects. It plays a variety of roles during tissue morphogenesis including inhibition of the planar cell polarity pathway and myoblast and osteoblast differentiation. It is also expressed in multiple myeloma and glioma in which it promotes tumorigenicity. Recombinant mouse sFRP-2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for sFRP-2 recombinant protein

Signal Transduction; Proteins

References

Alfaro, M.P. et al. (2010) J. Biol. Chem. 285:35645-35653; He, W. et al. (2010) Proc. Natl. Acad. Sci. 107:21110-21115

NCBI and Uniprot Product Information

• NCBI GI #: 6677895
• NCBI GeneID: 20319
• NCBI Accession #: NP_033170.1
• NCBI GenBank Nucleotide #: NM_009144.2
• UniProt Accession #: P97299; O08862; O35297
• Molecular Weight: 33,469 Da
• NCBI Official Full Name: secreted frizzled-related protein 2
• NCBI Official Synonym Full Names: secreted frizzled-related protein 2
• NCBI Official Symbol: Sfrp2
• NCBI Official Synonym Symbols: Sdf5; AI851596
• NCBI Protein Information: secreted frizzled-related protein 2
• UniProt Protein Name: Secreted frizzled-related protein 2
• UniProt Gene Name: Sfrp2
• UniProt Synonym Gene Names: Sarp1; Sdf5; sFRP-2; SARP-1

Uniprot Description

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.