Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat P2Y purinoceptor 14 (P2RY14) Standard Curve.)
Rat P2Y Purinoceptor 14 (P2RY14) ELISA Kit | P2RY14 elisa kit
Rat P2Y Purinoceptor 14 (P2RY14) ELISA Kit
Gene Names
P2ry14; Gpr105
Reactivity
Rat
Synonyms
P2Y Purinoceptor 14 (P2RY14); Rat P2Y Purinoceptor 14 (P2RY14) ELISA Kit; P2RY14; GPR105; KIAA0001; P2Y14; G protein coupled receptor for UDP-glucose; G protein-coupled receptor 105; P2Y purinoceptor 14; P2Y(14) receptor; P2Y14 receptor; P2RY14 elisa kit
Reactivity
Rat
Specificity
Rat P2Y purinoceptor 14 (P2RY14) ELISA Kit has high sensitivity and excellent specificity for detection of Rat P2RY14. No significant cross-reactivity or interference between Rat P2RY14 and analogues was observed.
Sequence Length
305
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat P2Y purinoceptor 14 (P2RY14) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat P2Y purinoceptor 14 (P2RY14) Standard Curve.)
Related Product Information for P2RY14 elisa kit
Description: This Rat P2Y purinoceptor 14 (P2RY14) ELISA Kit employs a two-site sandwich ELISA to quantitate P2RY14 in samples. An antibody specific for P2RY14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any P2RY14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for P2RY14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of P2RY14 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: P2Y purinoceptor 14 is a protein belongs to the family of G-protein coupled receptors, which contains several receptor subtypes with different pharmacological selectivity for various adenosine and uridine nucleotides. This receptor is a P2Y purinergic receptor for UDP-glucose and other UDP-sugars coupled to G-proteins. It has been implicated in extending the known immune system functions of P2Y receptors by participating in the regulation of the stem cell compartment, and it may also play a role in neuroimmune function. Two transcript variants encoding the same protein have been identified for this gene.The predicted 338-amino acid protein contains transmembrane domains and a tyrosine kinase phosphorylation site.
Background: P2Y purinoceptor 14 is a protein belongs to the family of G-protein coupled receptors, which contains several receptor subtypes with different pharmacological selectivity for various adenosine and uridine nucleotides. This receptor is a P2Y purinergic receptor for UDP-glucose and other UDP-sugars coupled to G-proteins. It has been implicated in extending the known immune system functions of P2Y receptors by participating in the regulation of the stem cell compartment, and it may also play a role in neuroimmune function. Two transcript variants encoding the same protein have been identified for this gene.The predicted 338-amino acid protein contains transmembrane domains and a tyrosine kinase phosphorylation site.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
P2Y purinoceptor 14
NCBI Official Synonym Full Names
purinergic receptor P2Y14
NCBI Official Symbol
P2ry14
NCBI Official Synonym Symbols
Gpr105
NCBI Protein Information
P2Y purinoceptor 14
UniProt Protein Name
P2Y purinoceptor 14
Protein Family
UniProt Gene Name
P2ry14
UniProt Synonym Gene Names
Gpr105; P2Y14
UniProt Entry Name
P2Y14_RAT
NCBI Description
G-protein coupled receptor; may play a role in neuroimmune function [RGD, Feb 2006]
Research Articles on P2RY14
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Product Notes
The Rat P2RY14 p2ry14 (Catalog #AAA9712149) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712149 ELISA Kit recognizes Rat P2RY14. It is sometimes possible for the material contained within the vial of "P2Y Purinoceptor 14 (P2RY14), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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