Mitogen Activated Kinase Kinase 1 Active Protein | MEK1 active protein

Recombinant Human Mitogen Activated Kinase Kinase 1

Cat. Num. AAA143393

• Gene Names: MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Mitogen Activated Kinase Kinase 1; Recombinant Human Mitogen Activated Kinase Kinase 1; MEK1 Human; Mitogen Activated Kinase Kinase 1 Human Recombinant; MEK1; MKK1; MAPKK1; MAP2K1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; EC 2.7.12.2; ERK activator kinase 1; MAPK/ERK kinase 1; PRKMK1; MEK1 active protein

• Host: Sf9 Insect Cells
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: MEK1 is supplied at a concentration of 0.09mg/ml in 40mM Tris, pH-8, 0.15M NaCl, 0.27M sucrose, 1mM DTT, 0.2mM PMSF, 1mM benzamidine, 0.1mM sodium vanadate and 0.03% Brij-35.; Sterile Filtered clear solution.
• Sequence Length: 393

• Source: Sf9 Insect Cells.
• Biological Activity: ~125-175 units/mg. One unit of MEK1 activity transfers 1 nmol of phosphate to ERK1/2 peptide per minute at 30 degree C in a reaction containing 100 uM ATP. Recombinant active MEK1 also phosphorylates ERK1, ERK2, and GSK-3?. Kinase activity may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application.
• Preparation and Storage: Store at 4°C if entire vial will be used within 1-2 weeks.; Store frozen at -20°C for longer periods of time.; Avoid multiple freeze-thaw cycles.

Related Product Information for MEK1 active protein

Description: MAP2K1 active Human Recombinant produced in Sf9 cells is a glycosylated, polypeptide chain containing amino acids 2-393 having a molecular mass of 47 kDa. MAP2K1 is fused to a polyhistidine tag and is purified by proprietary chromatographic techniques.

Introduction: MAP2K1 is a member of the dual specificity protein kinase family, which plays a role as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, are recognized as extracellular signal-regulated kinases (ERKs), that act as an integration position for multiple biochemical signals. MEK1 is located upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As a key player of MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. MAP2K1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a thr-glu-tyr sequence located in map kinases. MEK1 activates erk1 and erk2 map kinases.

Product Categories/Family for MEK1 active protein

Protein Kinases; Enzymes; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 5579478
• NCBI GeneID: 5604
• NCBI Accession #: NP_002746.1
• NCBI GenBank Nucleotide #: NM_002755.3
• UniProt Accession #: Q02750
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 1
• NCBI Official Symbol: MAP2K1
• NCBI Official Synonym Symbols: CFC3; MEK1; MKK1; MAPKK1; PRKMK1
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1; MAPKK 1; MEK 1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 1
• Protein Family: Meiosis-specific serine/threonine-protein kinase
• UniProt Gene Name: MAP2K1
• UniProt Synonym Gene Names: MEK1; PRKMK1; MAP kinase kinase 1; MAPKK 1; MKK1; MEK 1
• UniProt Entry Name: MP2K1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]

Uniprot Description

MEK1: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf, Mos and Cot kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK1 and ERK2. An essential component of MAP kinase signal transduction pathways involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Protein type: EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); Protein kinase, STE; Kinase, protein; STE group; STE7 family

Chromosomal Location of Human Ortholog: 15q22.1-q22.33

Cellular Component: dendrite cytoplasm; Golgi apparatus; microtubule; focal adhesion; mitochondrion; endoplasmic reticulum; early endosome; perikaryon; cell cortex; cytosol; perinuclear region of cytoplasm; axon; cytoplasm; late endosome; plasma membrane; microtubule organizing center; nucleus

Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activator activity; protein binding; Ras GTPase binding; receptor signaling protein tyrosine phosphatase activity; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; mitogen-activated protein kinase kinase kinase binding; ATP binding; protein kinase activity

Biological Process: axon guidance; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; protein heterooligomerization; activation of MAPK activity; negative regulation of homotypic cell-cell adhesion; response to glucocorticoid stimulus; stress-activated MAPK cascade; cell motility involved in cell locomotion; pathogenesis; toll-like receptor 3 signaling pathway; signal transduction; chemotaxis; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; neuron differentiation; negative regulation of cell proliferation; positive regulation of RNA elongation from RNA polymerase II promoter; small GTPase mediated signal transduction; vesicle transport along microtubule; response to axon injury; cell cycle arrest; toll-like receptor 4 signaling pathway; Golgi inheritance; epidermal growth factor receptor signaling pathway; mitosis; fibroblast growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; melanosome transport; toll-like receptor 2 signaling pathway; keratinocyte differentiation; MyD88-dependent toll-like receptor signaling pathway; regulation of stress-activated MAPK cascade; cell proliferation; Ras protein signal transduction; regulation of vascular smooth muscle contraction; toll-like receptor signaling pathway; insulin receptor signaling pathway; innate immune response; response to oxidative stress; toll-like receptor 9 signaling pathway; positive regulation of Ras protein signal transduction; positive regulation of cell differentiation; vascular endothelial growth factor receptor signaling pathway; cell motility; positive regulation of cell migration

Disease: Noonan Syndrome 1; Cardiofaciocutaneous Syndrome 3