Rabbit Sp3 Polyclonal Antibody | anti-Sp3 antibody

Anti-Sp3 Antibody

Cat. Num. AAA415454

• Gene Names: Sp3; D130027J01Rik
• Reactivity: Human, Mouse, Rat. Not yet tested in other species.
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: Sp3; Polyclonal Antibody; Anti-Sp3 Antibody; anti-Sp3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat. Not yet tested in other species.
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 688

• Applicable Applications for anti-Sp3 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA titer: 1:5,000-1:20,000; Immunohistochemistry: 1:200-500; Optimal dilutions/concentrations should be determined by the end user.
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Rabbit polyclonal Sp3 antibody was raised against a peptide at the intermediate residues of human Sp3 protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Immunohistochemistry (IHC)

Related Product Information for anti-Sp3 antibody

This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13. [provided by RefSeq, Feb 2010]

References

(1) Mora-Lopez,F., Pedreno-Horrillo,N., Delgado-Perez,L.,et al. Transcription of PRDM1, the master regulator for plasma cell differentiation, depends on an SP1/SP3/EGR-1 GC-box. Eur. J. Immunol. 38 (8), 2316-2324 (2008)

NCBI and Uniprot Product Information

• NCBI GI #: 62635492
• NCBI GeneID: 20687
• Molecular Weight: 76,729 Da
• NCBI Official Full Name: Sp3, partial
• NCBI Official Synonym Full Names: trans-acting transcription factor 3
• NCBI Official Symbol: Sp3
• NCBI Official Synonym Symbols: D130027J01Rik
• NCBI Protein Information: transcription factor Sp3
• UniProt Protein Name: Transcription factor Sp3
• Protein Family: Transcription factor
• UniProt Gene Name: Sp3
• UniProt Entry Name: SP3_MOUSE

NCBI Description

This gene product belongs to a family of Sp1 related transcription factors, which regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses transcription of numerous genes. Alternative splicing results in transcript variants encoding different isoforms, and one variant initiates translation from a non-AUG (AUA) codon. [provided by RefSeq, Jul 2008]

Uniprot Description

SP3: Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone- induction and house-keeping. Belongs to the Sp1 C2H2-type zinc-finger protein family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; C2H2-type zinc finger protein

Cellular Component: nucleoplasm; Golgi apparatus; transcriptional repressor complex; cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; nucleic acid binding; DNA binding; metal ion binding; double-stranded DNA binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; ossification; embryonic placenta development; transcription, DNA-dependent; granulocyte differentiation; monocyte differentiation; megakaryocyte differentiation; in utero embryonic development; embryonic skeletal development; positive regulation of transcription, DNA-dependent; embryonic process involved in female pregnancy; enucleate erythrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; liver development; trophectodermal cell differentiation; regulation of transcription, DNA-dependent; natural killer cell differentiation; B cell differentiation; erythrocyte differentiation; embryonic camera-type eye morphogenesis; negative regulation of transcription, DNA-dependent; T cell differentiation; lung development