Human Dual specificity mitogen-activated protein kinase kinase 1 ELISA Kit | MAP2K1 elisa kit

Human Dual specificity mitogen-activated protein kinase kinase 1 ELISA Kit

Cat. Num. AAA761359

• Gene Names: MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
• Reactivity: Human
• Synonyms: Dual specificity mitogen-activated protein kinase kinase 1; Human Dual specificity mitogen-activated protein kinase kinase 1 ELISA Kit; MAP2K1/MEK1/MKK1/dual specificity mitogen-activated protein kinase kinase 1/ERK activator kinase 1/MAP kinase kinase 1/MAPK/ERK kinase 1/MAPKK1/MEK1MAPKK 1/mitogen-activated protein kinase kinase 1/MKK1MEK 1/PRKMK1/protein kinase; mitogen-activated; kinase 1 (MAP kinase kinase 1); MAP2K1 elisa kit

• Reactivity: Human
• Sequence Length: 393

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for MAP2K1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MAP2K1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MAP2K1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MAP2K1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MAP2K1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 5579478
• NCBI GeneID: 5604
• NCBI Accession #: NP_002746.1
• NCBI GenBank Nucleotide #: NM_002755.3
• Molecular Weight: 40,764 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 1
• NCBI Official Symbol: MAP2K1
• NCBI Official Synonym Symbols: CFC3; MEK1; MKK1; MAPKK1; PRKMK1
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 1
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 1
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: MAP2K1
• UniProt Synonym Gene Names: MEK1; PRKMK1; MAP kinase kinase 1; MAPKK 1; MKK1; MEK 1
• UniProt Entry Name: MP2K1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]

Uniprot Description

MEK1: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf, Mos and Cot kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK1 and ERK2. An essential component of MAP kinase signal transduction pathways involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Protein type: EC 2.7.12.2; Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family

Chromosomal Location of Human Ortholog: 15q22.1-q22.33

Cellular Component: cytoplasm; cytosol; early endosome; endoplasmic reticulum; focal adhesion; Golgi apparatus; late endosome; microtubule organizing center; mitochondrion; nucleus; plasma membrane

Molecular Function: ATP binding; MAP kinase kinase activity; protein binding; protein C-terminus binding; protein kinase activity; protein N-terminus binding; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; protein-tyrosine kinase activity; receptor signaling protein tyrosine phosphatase activity

Biological Process: activation of MAPK activity; activation of MAPKK activity; axon guidance; Bergmann glial cell differentiation; cell cycle arrest; cell motility; cell motility involved in cell locomotion; cerebellar cortex formation; chemotaxis; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; heart development; innate immune response; insulin receptor signaling pathway; keratinocyte differentiation; MAPKKK cascade; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of cell proliferation; nerve growth factor receptor signaling pathway; peptidyl-tyrosine phosphorylation; positive regulation of axonogenesis; Ras protein signal transduction; regulation of apoptosis; regulation of axon regeneration; regulation of mitotic cell cycle; regulation of stress-activated MAPK cascade; signal transduction; small GTPase mediated signal transduction; stress-activated MAPK cascade; stress-activated protein kinase signaling pathway; thymus development; thyroid gland development; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway

Disease: Cardiofaciocutaneous Syndrome 3; Noonan Syndrome 1