Phosphodiesterase 7A Active Protein | PDE7A active protein

Phosphodiesterase 7A, Recombinant, Human (PDE7A)

Cat. Num. AAA638209

• Gene Names: PDE7A; HCP1; PDE7
• Purity: > = 44%
• Synonyms: Phosphodiesterase 7A; Recombinant; Human (PDE7A); PDE7A active protein

• Host: Sf9 insect cells
• Purity/Purification: > = 44%
• Form/Format: Supplied as a liquid in 40mM Tris-HCl ( pH 8.0), containing 100mM NaCl, 2.2mM KCl, 4mM glutathione, 0.04% Tween-20, 20% glycerol and 3mM DTT.

• Application Notes: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Specific Activity: > = 180pmol/min/ug (lot-specific)
• Unit Definition of Activity: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
• Assay for Activity: Activity is assayed using Ca. # 137347 (PDE7A Assay Kit, BioAssay™). Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200uM cAMP, 2.5kU 5'-nucleotidase, and serial dilutions of PDE7A at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
• Source: Baculovirus-infected Sf9 insect cells
• Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic applications.
• Preparation and Storage: Store at -80°C. Product is stable for 6 months -80°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.; Shipping: Ships with Dry Ice.

Related Product Information for PDE7A active protein

Human PDE7A GenBank Accession No. NM_002603), (a.a. 121-end) with N-terminal GST tag, expressed in a Baculovirus infected Sf9 cell expression system.

Product Categories/Family for PDE7A active protein

Molecular Biology; MB-Enzymes; Phosphodiesterase

NCBI and Uniprot Product Information

• NCBI GI #: 31043849
• NCBI GeneID: 5150
• UniProt Accession #: Q7Z7I0; Q13946
• Molecular Weight: 66kD
• NCBI Official Full Name: phosphodiesterase 7A
• NCBI Official Synonym Full Names: phosphodiesterase 7A
• NCBI Official Symbol: PDE7A
• NCBI Official Synonym Symbols: HCP1; PDE7
• NCBI Protein Information: high affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A; TM22; phosphodiesterase isozyme 7
• UniProt Protein Name: Phosphodiesterase 7A
• Protein Family: High affinity cAMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE7A
• UniProt Entry Name: Q7Z7I0_HUMAN

NCBI Description

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE7 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

PDE7A: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction. Belongs to the cyclic nucleotide phosphodiesterase family. PDE7 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Phosphodiesterase; EC 3.1.4.53; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 8q13

Cellular Component: cytosol

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; metal ion binding

Biological Process: cAMP catabolic process; signal transduction