Mouse IGF-1 ELISA Kit | IGF1 elisa kit

Mouse IGF-1 PicoKine ELISA Kit

Cat. Num. AAA175810

• Gene Names: Igf1; Igf-1; Igf-I; C730016P09Rik
• Reactivity: Mouse
• Synonyms: IGF-1; Mouse IGF-1 PicoKine ELISA Kit; Insulin-like growth factor I; insulin-like growth factor 1 (somatomedin C); IGF-I; Igf1; IGF1_HUMAN; Mechano growth factor; MGF; Somatomedin-C; IGF1 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse IGF-1
• Sequence Length: 143

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: < 5pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse IGF-1 PicoKine ELISA Kit standard curve )

Related Product Information for IGF1 elisa kit

Principle of the assay: mouse IGF-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGF-1has been precoated onto 96-well plates. Standards(E.coli,G49-A118) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF-1is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IGF-1 amount of sample captured in plate.
Background: Insulin-like growth factor 1 (IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Human IGF1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6 kD.1 The IGF1 gene, mapped on 12q22-q24.1, contains 5 exons. Exons 1-4encode the 195-amino acid precursor (IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide (IGF1A).2The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation.3 IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in aparacrine/autocrine fashion. Moreover, approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.

NCBI and Uniprot Product Information

• NCBI GeneID: 16000
• UniProt Accession #: E9PU89; P05017
• Molecular Weight: 15,992 Da
• NCBI Official Synonym Full Names: insulin-like growth factor 1
• NCBI Official Symbol: Igf1
• NCBI Official Synonym Symbols: Igf-1; Igf-I; C730016P09Rik
• NCBI Protein Information: insulin-like growth factor I; somatomedin
• UniProt Protein Name: Insulin-like growth factor I
• Protein Family: Insulin-like growth factor
• UniProt Gene Name: Igf1
• UniProt Entry Name: E9PU89_MOUSE