Specific Activity
(The specific activity of PAD4 was determined to be 4.1 nmol/min/mg as per activity assay protocol.)
PAD4 active protein
PAD4, Active
Purity
>95%
Synonyms
PAD4; Active; PADI4; PAD; PDI4; PDI5; PAD4 active protein
Host
Sf9 Insect Cells
Purity/Purification
>95%
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Concentration
0.1ug/ul (varies by lot)
Preparation and Storage
Store product at –70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Specific Activity
(The specific activity of PAD4 was determined to be 4.1 nmol/min/mg as per activity assay protocol.)
Specific Activity
(The specific activity of PAD4 was determined to be 4.1 nmol/min/mg as per activity assay protocol.)
SDS-Page
(The purity of PAD4 was determined to be >95% by densitometry. Approx. MW 96 kDa.)
SDS-Page
(The purity of PAD4 was determined to be >95% by densitometry. Approx. MW 96 kDa.)
Related Product Information for PAD4 active protein
Full length recombinant human protein expressed in Sf9 cells. Recombinant full-length human PAD4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is BC025718.
Scientific Background: PAD4 is a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions (1). PAD4 is essential for antibacterial innate immunity mediated by neutrophil extracellular traps (NETs) (2). PAD4 play a role in granulocyte and macrophage development leading to inflammation and immune response. PAD4 also mediates the gene expression by regulating arginine methylation and citrullination in histones (3).
Scientific Background: PAD4 is a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions (1). PAD4 is essential for antibacterial innate immunity mediated by neutrophil extracellular traps (NETs) (2). PAD4 play a role in granulocyte and macrophage development leading to inflammation and immune response. PAD4 also mediates the gene expression by regulating arginine methylation and citrullination in histones (3).
References
1. Nakashima, K. et.al: Molecular characterization of peptidylarginine deiminase in HL-60 cells induced by retinoic acid and 1-alpha,25-dihydroxyvitamin D(3). J. Biol. Chem. 274: 27786-27792, 1999.
2. Li, P.et.al: PAD4 is essential for antibacterial innate immunity mediated by neutrophil extracellular traps. J. Exp. Med. 207: 1853-1862, 2010.
3. Wang, Y. et.al: Human PAD4 regulates histone arginine methylation levels via demethylimination. Science 306: 279-283, 2004.
2. Li, P.et.al: PAD4 is essential for antibacterial innate immunity mediated by neutrophil extracellular traps. J. Exp. Med. 207: 1853-1862, 2010.
3. Wang, Y. et.al: Human PAD4 regulates histone arginine methylation levels via demethylimination. Science 306: 279-283, 2004.
NCBI and Uniprot Product Information
Protein Family
Similar Products
Product Notes
The PAD4 (Catalog #AAA515750) is an Active Protein produced from Sf9 Insect Cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PAD4, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
