Specific Activity
(The specific activity of IRAK1 was determined to be 27 nmol /min/mg as per activity assay protocol.)
IRAK1 active protein
IRAK1 (catalytic domain), Active
Purity
>85%
Synonyms
IRAK1; IRAK1 (catalytic domain); Active; IRAK; Pelle; IRAK1 active protein
Purity/Purification
>85%
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, and 25% glycerol.
Concentration
0.41 ug/ul (varies by lot)
Preparation and Storage
Stability:
1 year at -70 degree C from date of shipment.
Storage & Shipping:
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Shipping: Ships on Dry ice.
1 year at -70 degree C from date of shipment.
Storage & Shipping:
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Shipping: Ships on Dry ice.
Specific Activity
(The specific activity of IRAK1 was determined to be 27 nmol /min/mg as per activity assay protocol.)
Specific Activity
(The specific activity of IRAK1 was determined to be 27 nmol /min/mg as per activity assay protocol.)
SDS-Page
(The purity was determined to be >85% by densitometry. Approx. MW 85-100 kDa.)
SDS-Page
(The purity was determined to be >85% by densitometry. Approx. MW 85-100 kDa.)
Related Product Information for IRAK1 active protein
Recombinant human IRAK1 (194-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_001569.
IRAK1 encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. The exposure of HeLa cells or human embryonic kidney cells overexpressing IL1R to IL1 caused rapid association of IRAK with the IL1R complex and phosphorylation of IRAK (1). IRAK1 is partially responsible for IL1-induced upregulation of the transcription factor NFkappa B. IRAK1 as a risk gene with critical role in the pathogenesis of systemic lupus erythematosus.
IRAK1 encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. The exposure of HeLa cells or human embryonic kidney cells overexpressing IL1R to IL1 caused rapid association of IRAK with the IL1R complex and phosphorylation of IRAK (1). IRAK1 is partially responsible for IL1-induced upregulation of the transcription factor NFkappa B. IRAK1 as a risk gene with critical role in the pathogenesis of systemic lupus erythematosus.
References
1. Cao, Z.et.al: IRAK: a kinase associated with the interleukin-1 receptor. Science 271: 1128-1131, 1996.
2. Jacob, C. O. et.al: Identification of IRAK1 as a risk gene with critical role in the pathogenesis of systemic lupus erythematosus. Proc. Nat. Acad. Sci. 106: 6256-6261, 2009.
2. Jacob, C. O. et.al: Identification of IRAK1 as a risk gene with critical role in the pathogenesis of systemic lupus erythematosus. Proc. Nat. Acad. Sci. 106: 6256-6261, 2009.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Protein Family
Similar Products
Product Notes
The IRAK1 (Catalog #AAA515711) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "IRAK1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
