PDE7B active protein

PDE7B, Active

Cat. Num. AAA515638

• Gene Names: PDE7B; bA472E5.1
• Purity: >75%
• Synonyms: PDE7B; Active; PDE7B active protein

• Host: Sf9 cells
• Purity/Purification: >75%
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Concentration: 0.1 ug/ul (varies by lot)

• Specific Activity: 25 nmol/min/mg
• Preparation and Storage: Shipping: Dry Ice; Store product at -70 degree C.; For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.; For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Specific Activity

Purity

Related Product Information for PDE7B active protein

Description: Full-length recombinant human PDE7B was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: PDE7B is a cAMP-specific phosphodiesterase and a member of the cyclic nucleotide phosphodiesterase family that catalyzes the hydrolysis of cAMP to the corresponding 5'-monophosphate and provides a mechanism to downregulate cAMP signaling (1). PDE7B is over-expressed in chronic lymphocytic leukemia and inhibition of PDE7B leads to induction of apoptosis (2). PDE7B has been identified as a candidate gene for measuring treatment response to risperidone and may confer susceptibility to schizophrenia.

Product Categories/Family for PDE7B active protein

Enzymes; Active Phosphodiesterases

References

1. Hetman, J. M. et.al: Cloning and characterization of PDE7B, a cAMP-specific phosphodiesterase. Proc. Nat. Acad. Sci. 97: 472-476, 2000. 2. Ikeda, M. et al: Identification of novel candidate genes for treatment response to risperidone and susceptibility for schizophrenia: integrated analysis among pharmacogenomics, mouse expression, and genetic case-control association approaches. Biol Psychiatry. 2010 Feb 1;67 (3):263-9.

NCBI and Uniprot Product Information

• NCBI GI #: 57242789
• NCBI GeneID: 27115
• NCBI Accession #: NM_018945.3
• NCBI GenBank Nucleotide #: NM_018945
• UniProt Accession #: Q9NP56; Q5W154
• Molecular Weight: ~73 kDa
• NCBI Official Full Name: Homo sapiens phosphodiesterase 7B (PDE7B), mRNA
• NCBI Official Synonym Full Names: phosphodiesterase 7B
• NCBI Official Symbol: PDE7B
• NCBI Official Synonym Symbols: bA472E5.1
• NCBI Protein Information: cAMP-specific 3',5'-cyclic phosphodiesterase 7B
• UniProt Protein Name: cAMP-specific 3',5'-cyclic phosphodiesterase 7B
• Protein Family: cAMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE7B

NCBI Description

The 3',5'-cyclic nucleotides cAMP and cGMP function as second messengers in a wide variety of signal transduction pathways. 3',5'-cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of cAMP and cGMP to the corresponding 5'-monophosphates and provide a mechanism to downregulate cAMP and cGMP signaling. This gene encodes a cAMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family.[provided by RefSeq, Apr 2009]

Uniprot Description

PDE7B: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in the control of cAMP-mediated neural activity and cAMP metabolism in the brain. Belongs to the cyclic nucleotide phosphodiesterase family. PDE7 subfamily.

Protein type: EC 3.1.4.53; Nucleotide Metabolism - purine; Phosphodiesterase

Chromosomal Location of Human Ortholog: 6q23.3

Cellular Component: cytosol

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity

Biological Process: synaptic transmission