Carboxyl Ester Lipase Active Protein | mCEL active protein

Recombinant Mouse Carboxyl Ester Lipase

Cat. Num. AAA141457

• Gene Names: Cel; BAL; BSSL; 1810036E18Rik
• Purity: >90.0% as determined by SDS-PAGE.
• Synonyms: Carboxyl Ester Lipase; Recombinant Mouse Carboxyl Ester Lipase; Bile salt-activated lipase; BAL; EC 3.1.1.13; EC 3.1.1.3; Bile salt-stimulated lipase; BSSL; Bucelipase; Carboxyl ester lipase; Cholesterol esterase; Pancreatic lysophospholipase; Sterol esterase; CEL; FAP; BSDL; CELL; FAPP; LIPA; Cease; MODY8; CEL Mouse; Carboxyl Ester Lipase Mouse Recombinant; mCEL active protein

• Host: Sf9, Baculovirus Cells
• Purity/Purification: >90.0% as determined by SDS-PAGE.
• Form/Format: Sterile filtered colorless solution.; The CEL solution (0.5 mg/ml) contains 10% Glycerol and Phosphate-Buffered Saline (pH 7.4).
• Sequence: Amino acid: AKLGAVYTEG GFVEGVNKKL SLLGGDSVDI FKGIPFATAK TLENPQRHPG WQGTLKATNFKKRCLQATIT QDNTYGQEDC LYLNIWVPQG RKQVSHNLPV MVWIYGGAFL MGSGQGANFLKNYLYDGEEI ATRGNVIVVT FNYRVGPLGF LSTGDANLPG NFGLRDQHMA IAWVKRNIAAFGGDPDNITI FGESAGAASV SLQTLSPYNK GLIRRAISQS GMALSPWAIQ KNPLFWAKTIAKKVGCPTED TGKMAACLKI TDPRALTLAY KLPVKKQEYP VVHYLAFIPV IDGDFIPDDPINLYNNTADI DYIAGINNMD GHLFATIDVP AVDKTKQTVT EEDFYRLVSG HTVAKGLKGAQATFDIYTES WAQDPSQENM KKTVVAFETD VLFLIPTEIA LAQHKAHAKS AKTYSYLFSHPSRMPIYPKW MGADHADDLQ YVFGKPFATP LGYRPQDRAV SKAMIAYWTN FARSGDPNMGNSPVPTHWYP YTLENGNYLD ITKTITSASM KEHLREKFLK FWAVTFEVLP TVTGDQDTLTPPEDDSEVAP DPPSDDSQVV PVPPTDDSVE AQMPATIGFH HHHHH
• Sequence Length: 599

• Biological Activity: Specific activity is > 100,000 pmol/min/ug. Measured by the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl butyrate to p-nitrophenol per minute at pH7.5 at 25 degree C.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks.; Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).; Avoid multiple freeze-thaw cycles.

Related Product Information for mCEL active protein

Introduction: Carboxyl ester lipase also known as CEL, formely called cholesterol esterase or bile salt-stimulated lipase, is an enzyme with lipolytic capablity of hydrolyzing cholesteryl esters, tri-, di-, and mono- phospholipids, acylglycerol, ceramide and lysophospholipids. The carboxyl terminus of the enzyme controls enzymatic activity by creating hydrogen bonds with the surface loop to partlyshield the active site. The active catalytic site triad of serine-histidine-aspartate is centrally located in the enzyme structure and is partly covered by a surface loop. Bile salt binding to the loop domain set free the active site for accessibility by water-insoluble substrates. CEL is produced mainly in the pancreas and lactating mammary gland, thus the protein is also expressed in liver, macrophages, and in the vessel wall.

Description: CEL Mouse produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 585 amino acids (21-599 aa) and having a molecular mass of 64.5kDa.CEL is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.

Product Categories/Family for mCEL active protein

ENZYMES

NCBI and Uniprot Product Information

• NCBI GI #: 1046363
• NCBI GeneID: 12613
• UniProt Accession #: Q64285
• NCBI Official Full Name: cholesterol esterase
• NCBI Official Synonym Full Names: carboxyl ester lipase
• NCBI Official Symbol: Cel
• NCBI Official Synonym Symbols: BAL; BSSL; 1810036E18Rik
• NCBI Protein Information: bile salt-activated lipase
• UniProt Protein Name: Bile salt-activated lipase
• UniProt Gene Name: Cel
• UniProt Synonym Gene Names: Lip1; BAL; BSSL
• UniProt Entry Name: CEL_MOUSE

Uniprot Description

CEL: Catalyzes fat and vitamin absorption. Acts in concert with pancreatic lipase and colipase for the complete digestion of dietary triglycerides. Defects in CEL are a cause of maturity-onset diabetes of the young type 8 with exocrine dysfunction (MODY8); also known as diabetes and pancreatic exocrine dysfunction (DPED). MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Lipid Metabolism - glycerolipid; Lipase; Lipid Metabolism - steroid biosynthesis; Secreted; EC 3.1.1.3; EC 3.1.1.13

Cellular Component: Golgi apparatus; extracellular space; protein complex; rough endoplasmic reticulum; intracellular membrane-bound organelle; cytoplasm; extracellular region; cytosol; lipid raft

Molecular Function: sterol esterase activity; retinyl-palmitate esterase activity; triacylglycerol lipase activity; hydrolase activity; protein complex binding; glycosphingolipid binding; lysophospholipase activity

Biological Process: retinol metabolic process; ceramide catabolic process; lipid metabolic process; pancreatic juice secretion; cholesterol catabolic process; lipid catabolic process