Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Chymotrypsin-like elastase family member 2A (CEL) Standard Curve.)
Mouse Chymotrypsin-Like Elastase Family Member 2A (CEL) ELISA Kit | CEL elisa kit
Mouse Chymotrypsin-Like Elastase Family Member 2A (CEL) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Chymotrypsin-like elastase family member 2A (CEL) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Chymotrypsin-like elastase family member 2A (CEL) Standard Curve.)
Background: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2A is secreted from the pancreas as a zymogen. In other species, elastase 2A has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues. Clinical literature that describes human elastase 1 activity in the pancreas is actually referring to elastase 2A. The deduced 269-amino acid protein shares 55 to 84% identity with other mammalian elastases and 39% identity with bovine chymotrypsin A. Most homology resides in the N-terminal sequence and in the C-terminal half of the protein.
NCBI and Uniprot Product Information
Uniprot Description
CEL: Catalyzes fat and vitamin absorption. Acts in concert with pancreatic lipase and colipase for the complete digestion of dietary triglycerides. Defects in CEL are a cause of maturity-onset diabetes of the young type 8 with exocrine dysfunction (MODY8); also known as diabetes and pancreatic exocrine dysfunction (DPED). MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Lipid Metabolism - glycerolipid; Lipase; Lipid Metabolism - steroid biosynthesis; Secreted; EC 3.1.1.3; EC 3.1.1.13
Cellular Component: Golgi apparatus; extracellular space; protein complex; rough endoplasmic reticulum; intracellular membrane-bound organelle; cytoplasm; extracellular region; cytosol; lipid raft
Molecular Function: sterol esterase activity; retinyl-palmitate esterase activity; triacylglycerol lipase activity; hydrolase activity; protein complex binding; glycosphingolipid binding; lysophospholipase activity
Biological Process: retinol metabolic process; ceramide catabolic process; lipid metabolic process; pancreatic juice secretion; cholesterol catabolic process; lipid catabolic process
Research Articles on CEL
Similar Products
Product Notes
The Mouse CEL cel (Catalog #AAA9718009) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718009 ELISA Kit recognizes Mouse CEL. It is sometimes possible for the material contained within the vial of "Chymotrypsin-Like Elastase Family Member 2A (CEL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
