Granzyme-B Active Protein | GZMB active protein

Recombinant Human Granzyme-B, Sf9

Cat. Num. AAA141253

• Gene Names: GZMB; C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Granzyme-B; Recombinant Human Granzyme-B; Sf9; Sf9 Human Recombinant; Granzyme B; T-Cell Serine Protease 1-3E; Cathepsin G-Like 1; Granzyme B (Granzyme 2; Cytotoxic T-Lymphocyte-Associated Serine Esterase 1; Cytotoxic T-Lymphocyte Proteinase 2; Cytotoxic Serine Protease B; Human Lymphocyte Protein; Fragmentin 2; EC 3.4.21.79; CTSGL1; CTLA1; CSPB; CGL1; SECT; C11; HLP; Lymphocyte Protease; Fragmentin-2; Granzyme 2; Granzyme-2; EC 3.4.21; CTLA-1; CGL-1; CSP-B; CCPI; GRB; GZMB Human; sf9; GZMB active protein

• Host: Sf9, Baculovirus cells
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: Sterile filtered colorless solution.; Protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4),20% glycerol and 1mM DTT.
• Sequence: GEIIGGHEAK PHSRPYMAYL MIWDQKSLKR CGGFLIRDDF VLTAAHCWGS SINVTLGAHN IKEQEPTQQF IPVKRPIPHP AYNPKNFSND IMLLQLERKA KRTRAVQPLR LPSNKAQVKP GQTCSVAGWG QTAPLGKHSH TLQEVKMTVQ EDRKCESDLR HYYDSTIELC VGDPEIKKTS FKGDSGGPLV CNKVAQGIVS YGRNNGMPPR ACTKVSSFVH WIKKTMKRYH HHHHH
• Sequence Length: 247

• Biological Activity: Specific activity is > 7,000 pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time.; For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).; Avoid multiple freeze-thaw cycles.

Related Product Information for GZMB active protein

Description: GZMB produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 235 amino acids (19-247a.a.) and having a molecular mass of 26.5kDa. (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). GZMB is expressed with an 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Introductions: Granzyme-B (GZMB) is essential for target cell lysis in cell-mediated immune responses. GZMB is related to an activation cascade of caspases responsible for apoptosis execution. GZMB cuts caspase-3, -7, -9 and 10 to activate enzymes mediating apoptosis.

Product Categories/Family for GZMB active protein

Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 317373361
• NCBI GeneID: 3002
• UniProt Accession #: P10144; Q8N1D2; Q9UCC1
• Molecular Weight: 27,716 Da
• NCBI Official Full Name: Granzyme B
• NCBI Official Synonym Full Names: granzyme B
• NCBI Official Symbol: GZMB
• NCBI Official Synonym Symbols: C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• NCBI Protein Information: granzyme B
• UniProt Protein Name: Granzyme B
• Protein Family: Granzyme
• UniProt Gene Name: GZMB
• UniProt Synonym Gene Names: CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP

NCBI Description

This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016]

Uniprot Description

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.