Western Blot (WB)
(Anti-CISH antibody, MBS175768, Western blottingAll lanes: Anti CISH (MBS175768) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: Human Placenta Tissue Lysate at 50ugLane 4: A431 Whole Cell Lysate at 40ugLane 5: SMMC Whole Cell Lysate at 40ugLane 6: HELA Whole Cell Lysate at 40ugLane 7: COLO320 Whole Cell Lysate at 40ugLane 8: MM231 Whole Cell Lysate at 40ugPredicted bind size: 29KDObserved bind size: 29KD)
Rabbit CISH Polyclonal Antibody | anti-CISH antibody
Anti-CISH antibody
Western Blot (WB)
(Anti-CISH antibody, MBS175768, Western blottingAll lanes: Anti CISH (MBS175768) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: Human Placenta Tissue Lysate at 50ugLane 4: A431 Whole Cell Lysate at 40ugLane 5: SMMC Whole Cell Lysate at 40ugLane 6: HELA Whole Cell Lysate at 40ugLane 7: COLO320 Whole Cell Lysate at 40ugLane 8: MM231 Whole Cell Lysate at 40ugPredicted bind size: 29KDObserved bind size: 29KD)
Western Blot (WB)
(Anti-CISH antibody, MBS175768, Western blottingAll lanes: Anti CISH (MBS175768) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: Human Placenta Tissue Lysate at 50ugLane 4: A431 Whole Cell Lysate at 40ugLane 5: SMMC Whole Cell Lysate at 40ugLane 6: HELA Whole Cell Lysate at 40ugLane 7: COLO320 Whole Cell Lysate at 40ugLane 8: MM231 Whole Cell Lysate at 40ugPredicted bind size: 29KDObserved bind size: 29KD)
Immunohistochemistry (IHC)
(Anti-CISH antibody, MBS175768, IHC(P)IHC(P): Human Lung Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-CISH antibody, MBS175768, IHC(P)IHC(P): Human Lung Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-CISH antibody, MBS175768, IHC(P)IHC(P): Rat Kidney Tissue )
Immunohistochemistry (IHC)
(Anti-CISH antibody, MBS175768, IHC(P)IHC(P): Rat Kidney Tissue )
Background: CISH(cytokine inducible SH2-containing protein),also called CIS, CIS-1, G18, SOCS, is an important negative regulator for inflammatory signaling and belongs to the suppressors of cytokine signaling(SOCS) family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. CISH controls interleukin-2 signaling, and variations of CISH with certain SNPs are associated with susceptibility to bacteremia, tuberculosis and malaria. The human CISH gene is mapped to chromosome 3p21.3 by FISH. The mouse gene is tightly linked to the Gnai2 gene on chromosome 9, a region syntenic to human chromosome 3p21. CIS expression was upregulated by lipopolysaccharide(LPS) or Cryptosporidium parvum exposure, and this upregulation involved downregulation of MIR98 and LET7, which relieved MIR98- and LET7-mediated translational repression of CIS. Gain- and loss-of-function studies showed that CIS accelerated degradation of IKBA and enhanced NFKB activation in cholangiocytes in response to LPS stimulation or C parvum exposure.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
Uniprot Description
CISH: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate- recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Stably associated with the tyrosine-phosphorylated IL3 receptor beta chain and tyrosine-phosphorylated EPO receptor (EPOR). By a subset of cytokines including EPO/erythropoietin. Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct. 3 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 3p21.3
Cellular Component: cytoplasm; plasma membrane; cytosol
Molecular Function: protein binding; protein kinase inhibitor activity
Biological Process: cytokine and chemokine mediated signaling pathway; protein kinase C activation; negative regulation of insulin receptor signaling pathway; negative regulation of protein kinase activity; protein ubiquitination; regulation of cell growth; negative regulation of JAK-STAT cascade
Disease: Mycobacterium Tuberculosis, Susceptibility To; Bacteremia, Susceptibility To, 2; Malaria, Susceptibility To
Research Articles on CISH
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Product Notes
The CISH cish (Catalog #AAA175768) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CISH antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CISH can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the CISH cish for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CISH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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