Carboxylesterase 1D Active Protein | CES1D active protein
Recombinant Mouse Carboxylesterase 1D
Gene Names
Ces1d; TGH; Ces3
Purity
Greater than 90.0% as determined by SDS-PAGE.
Synonyms
Carboxylesterase 1D; Recombinant Mouse Carboxylesterase 1D; CES1D Mouse; Carboxylesterase 3 (EC: 3.1.1.1; EC: 3.1.1.67); Fatty acid ethyl ester synthase; FAEE synthase; Triacylglycerol hydrolase; TGH; CES1D; Carboxylesterase 1D Mouse Recombinant; CES1D active protein
Host
Sf9, Baculovirus cells
Purity/Purification
Greater than 90.0% as determined by SDS-PAGE.
Form/Format
Sterile filtered colorless solution.
CES1D protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
CES1D protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Sequence
YPSSPPVVNT VKGKVLGKYV NLEGFTQPVA VFLGVPFAKP PLGSLRFAPP QPAEPWSFVK NTTSYPPMCS QDAVGGQVLS ELFTNRKENI PLQFSEDCLY LNIYTPADLT KNSRLPVMVW IHGGGLVVGG ASTYDGLALS AHENVVVVTI QYRLGIWGFF STGDEHSRGN WGHLDQVAALRWVQDNIANF GGNPGSVTIF GESAGGFSVS VLVLSPLAKN LFHRAISESG VSLTAALITT DVKPIAGLVA TLSGCKTTTS AVMVHCLRQK TEDELLETSL KLNLFKLDLL GNPKESYPFL PTVIDGVVLP KAPEEILAEK SFSTVPYIVG INKQEFGWII PTLMGYPLAE GKLDQKTANSLLWKSYPTLK ISENMIPVVA EKYLGGTDDL TKKKDLFQDL MADVVFGVPS VIVSRSHRDA GASTYMYEFE YRPSFVSAMR PKAVIGDHGD EIFSVFGSPF LKDGASEEET NLSKMVMKFW ANFARNGNPN GGGLPHWPEY DQKEGYLKIG ASTQAAQRLK DKEVSFWAEL RAKESAQRPSHREHVELLEH HHHHH.
Sequence Length
565
Biological Activity
Specific activity is > 80, 000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to pnitrophenol per minute at pH 7.5 at 37C.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Related Product Information for CES1D active protein
Description: CES1D Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 555 amino acids (19-565 a.a) and having a molecular mass of 60.9kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). CES1D is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Introduction: Carboxylesterase 1D, also known as CES1D is part of a big family of carboxylesterases which are responsible for the hydrolysis of ester in addition to amide bonds. CES1D is the principle lipase of white adipose tissue fat cake extracts. Partially purified white adipose tissue Ces1d had lipase activity in addition to lesser but detectable neutral cholesteryl ester hydrolase activity. CES1D demonstrates low catalytic efficiency for hydrolysis of CPT-11, a prodrugs for camptothecin used in cancer therapeutics.
Introduction: Carboxylesterase 1D, also known as CES1D is part of a big family of carboxylesterases which are responsible for the hydrolysis of ester in addition to amide bonds. CES1D is the principle lipase of white adipose tissue fat cake extracts. Partially purified white adipose tissue Ces1d had lipase activity in addition to lesser but detectable neutral cholesteryl ester hydrolase activity. CES1D demonstrates low catalytic efficiency for hydrolysis of CPT-11, a prodrugs for camptothecin used in cancer therapeutics.
Product Categories/Family for CES1D active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,788 Da
NCBI Official Full Name
carboxylesterase 1D
NCBI Official Synonym Full Names
carboxylesterase 1D
NCBI Official Symbol
Ces1d
NCBI Official Synonym Symbols
TGH; Ces3
NCBI Protein Information
carboxylesterase 1D
UniProt Protein Name
Carboxylesterase 1D
Protein Family
UniProt Gene Name
Ces1d
UniProt Synonym Gene Names
; FAEE synthase; TGH
Uniprot Description
CES1D:
Protein type: EC 3.1.1.1; EC 3.1.1.67
Chromosomal Location of Human Ortholog: 8|8 C5
Cellular Component: cytosol; endoplasmic reticulum; endoplasmic reticulum lumen; extracellular space; lipid particle
Molecular Function: sterol esterase activity; triacylglycerol lipase activity
Biological Process: acyl-CoA metabolic process; acylglycerol catabolic process; cholesterol biosynthetic process; epithelial cell differentiation; lipid catabolic process; medium-chain fatty acid metabolic process; response to toxin; short-chain fatty acid catabolic process
Research Articles on CES1D
Similar Products
Product Notes
The CES1D ces1d (Catalog #AAA146778) is an Active Protein produced from Sf9, Baculovirus cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: YPSSPPVVNT VKGKVLGKYV NLEGFTQPVA VFLGVPFAKP PLGSLRFAPP QPAEPWSFVK NTTSYPPMCS QDAVGGQVLS ELFTNRKENI PLQFSEDCLY LNIYTPADLT KNSRLPVMVW IHGGGLVVGG ASTYDGLALS AHENVVVVTI QYRLGIWGFF STGDEHSRGN WGHLDQVAAL RWVQDNIANF GGNPGSVTIF GESAGGFSVS VLVLSPLAKN LFHRAISESG VSLTAALITT DVKPIAGLVA TLSGCKTTTS AVMVHCLRQK TEDELLETSL KLNLFKLDLL GNPKESYPFL PTVIDGVVLP KAPEEILAEK SFSTVPYIVG INKQEFGWII PTLMGYPLAE GKLDQKTANS LLWKSYPTLK ISENMIPVVA EKYLGGTDDL TKKKDLFQDL MADVVFGVPS VIVSRSHRDA GASTYMYEFE YRPSFVSAMR PKAVIGDHGD EIFSVFGSPF LKDGASEEET NLSKMVMKFW ANFARNGNPN GGGLPHWPEY DQKEGYLKIG ASTQAAQRLK DKEVSFWAEL RAKESAQRPS HREHVEL LEH HHHHH. It is sometimes possible for the material contained within the vial of "Carboxylesterase 1D, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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