Rabbit NOS3 Polyclonal Antibody | anti-NOS3 antibody

NOS3 (Phospho-S1177) Polyclonal Antibody

Cat. Num. AAA3005392

• Gene Names: NOS3; eNOS; ECNOS
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: NOS3; Polyclonal Antibody; NOS3 (Phospho-S1177) Polyclonal Antibody; Nitric oxide synthase; endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII; anti-NOS3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-NOS3 (S1177) polyclonal antibody detects endogenous levels of NOS3 protein when phosphorylated at Ser1177.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1203

• Applicable Applications for anti-NOS3 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500-1:1000; ICC: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human NOS3 around the phosphorylation site of Serine 1177.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-NOS3 (S1177) polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysateLane2:sp2/0 cell lysateLane3:H9C2 cell lysate)

Western Blot (WB)

(Western Blot (WB) analysis of p-NOS3 (S1177) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:HEK293T treated with PMA (60ng/ml, 5min) whole cell lysate (40ug) Lane3:HEK293T treated with PMA (60ng/ml, 15min) whole cell lysate (40ug) Lane4:HEK293T treated with PMA (60ng/ml, 30min) whole cell lysate (40ug))

Related Product Information for anti-NOS3 antibody

NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS).eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.

NCBI and Uniprot Product Information

• NCBI GI #: 40254422
• NCBI GeneID: 4846
• NCBI Accession #: NP_000594.2
• NCBI GenBank Nucleotide #: NP_000594.2
• UniProt Accession #: P29474
• Molecular Weight: ~ 140kDa
• NCBI Official Full Name: nitric oxide synthase, endothelial isoform 1
• NCBI Official Synonym Full Names: nitric oxide synthase 3
• NCBI Official Symbol: NOS3
• NCBI Official Synonym Symbols: eNOS; ECNOS
• NCBI Protein Information: nitric oxide synthase, endothelial
• UniProt Protein Name: Nitric oxide synthase, endothelial
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: NOS3
• UniProt Synonym Gene Names: cNOS; eNOS; NOSIII
• UniProt Entry Name: NOS3_HUMAN

NCBI Description

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]

Uniprot Description

eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.

Protein type: EC 1.14.13.39; Oxidoreductase; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: Golgi membrane; cytoskeleton; cytoplasm; plasma membrane; caveola; nucleus; cytosol

Molecular Function: calmodulin binding; actin monomer binding; protein binding; FAD binding; FMN binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity; iron ion binding; heme binding; NADP binding; cadmium ion binding

Biological Process: regulation of systemic arterial blood pressure by endothelin; negative regulation of potassium ion transport; negative regulation of muscle hyperplasia; positive regulation of vasodilation; nitric oxide metabolic process; negative regulation of cell proliferation; negative regulation of hydrolase activity; regulation of blood pressure; regulation of the force of heart contraction by chemical signal; lipopolysaccharide-mediated signaling pathway; angiogenesis; negative regulation of blood pressure; regulation of blood vessel size; nitric oxide biosynthetic process; mitochondrion organization and biogenesis; interaction with host; negative regulation of calcium ion transport; in utero embryonic development; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; ovulation from ovarian follicle; positive regulation of angiogenesis; response to heat; endothelial cell migration; arginine catabolic process; blood vessel remodeling; regulation of nitric-oxide synthase activity; vascular endothelial growth factor receptor signaling pathway; blood coagulation; nitric oxide mediated signal transduction; lung development; smooth muscle hyperplasia

Disease: Preeclampsia/eclampsia 1; Alzheimer Disease; Hypertension, Essential; Stroke, Ischemic