SerpinI1 / Neuroserpin Recombinant Protein | SERPINI1 recombinant protein

Recombinant Human SerpinI1 / Neuroserpin Protein (His tag)

Cat. Num. AAA2545911

• Gene Names: SERPINI1; PI12; neuroserpin
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: SerpinI1 / Neuroserpin; Recombinant Human SerpinI1 / Neuroserpin Protein (His tag); SERPINI1; DKFZp781N13156; PI12; neuroserpin; SERPINI1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 410

• Application Notes: The secreted recombinant human SERPINI1 consists of 405 amino acids after removal of the signal peptide and has a calculated molecular mass of 46 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh SERPINI1 is approximately 50-55 kDa due to glycosylation.
• Predicted N Terminal: Thr 17
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for SERPINI1 recombinant protein

Background: Neuroserpin, also known as Protease inhibitor 12 and SERPINI1, is a secreted protein which belongs to the serpin family. Neuroserpin is a serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. Serine protease inhibitors of the serpin superfamily are involved in many cellular processes. Neuroserpin was first identified as a protein secreted from the axons of dorsal root ganglion neurons. Neuroserpin is predominantly expressed in the brain, and is expressed in the late stages of neurogenesis during the process of synapse formation. Overexpression of neuroserpin in an anterior pituitary corticotroph cell line results in the extension of neurite-like processes, suggesting that neuroserpin may play a role in cell communication, cell adhesion, and/or cell migration. Neuroserpin may be involved in the formation or reorganization of synaptic connections, as well as synaptic plasticity in the adult nervous system. Neuroserpin may also protect neurons from cell damage by tissue-type plasminogen activator. Defects of neuroserpin are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B).

Description: A DNA sequence encoding the human SERPINI1 (NP_005016.1) (Met 1-Leu 410) was expressed, with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 4826904
• NCBI GeneID: 5274
• NCBI Accession #: NP_005016.1
• NCBI GenBank Nucleotide #: NM_005025.4
• UniProt Accession #: Q99574; Q6AHZ4; A8K217; D3DNP1
• Molecular Weight: 46,427 Da
• NCBI Official Full Name: neuroserpin
• NCBI Official Synonym Full Names: serpin family I member 1
• NCBI Official Symbol: SERPINI1
• NCBI Official Synonym Symbols: PI12; neuroserpin
• NCBI Protein Information: neuroserpin
• UniProt Protein Name: Neuroserpin
• Protein Family: Neuroserpin
• UniProt Gene Name: SERPINI1
• UniProt Synonym Gene Names: PI12; PI-12

NCBI Description

This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The protein is primarily secreted by axons in the brain, and preferentially reacts with and inhibits tissue-type plasminogen activator. It is thought to play a role in the regulation of axonal growth and the development of synaptic plasticity. Mutations in this gene result in familial encephalopathy with neuroserpin inclusion bodies (FENIB), which is a dominantly inherited form of familial encephalopathy and epilepsy characterized by the accumulation of mutant neuroserpin polymers. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

SERPINI1: Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator. Defects in SERPINI1 are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B). FEN1B is characterized clinically as an autosomal dominantly inherited dementia, histologically by unique neuronal inclusion bodies and biochemically by polymers of neuroserpin. Belongs to the serpin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q26.1

Cellular Component: extracellular space

Biological Process: central nervous system development; peripheral nervous system development

Disease: Encephalopathy, Familial, With Neuroserpin Inclusion Bodies